BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血清总铁结合能力（TIBC）指血清中转铁蛋白可结合铁的能力，其水平与缺铁性贫血、急性肝炎等疾病的发生密切相关。本产品提供了一种简便、灵敏、快速的TIBC检测方法，适用于血清（浆）样本。检测原理如下：Fe²⁺与菲洛嗪反应生成紫红色化合物，该产物在562 nm处具有特征吸收峰。在碱性条件下，血清转铁蛋白可与Fe³⁺结合；剩余未结合的Fe³⁺被还原为Fe²⁺，此时测得的吸光度A₁与未结合Fe³⁺的量成正比。酸化处理后，转铁蛋白结合的Fe³⁺被释放并进一步还原为Fe²⁺，此时测得的吸光度A₂与总Fe³⁺的量成正比。A₂与A₁的差值（A₂−A₁）与TIBC浓度呈正比。
Serum total iron‑binding capacity (TIBC) refers to the ability of serum transferrin to bind iron. Its level is closely associated with the occurrence of diseases such as iron deficiency anemia and acute hepatitis. This product provides a simple, sensitive, and rapid method for TIBC determination, suitable for serum or plasma samples. The detection principle is as follows: Fe²⁺ reacts with ferrozine to form a purple‑red compound, which exhibits a characteristic absorption peak at 562 nm. Under alkaline conditions, serum transferrin binds to Fe³⁺; the remaining unbound Fe³⁺ is reduced to Fe²⁺, and the measured absorbance A₁ is proportional to the amount of unbound Fe³⁺. After acidification treatment, the transferrin‑bound Fe³⁺ is released and further reduced to Fe²⁺, and the measured absorbance A₂ is proportional to the amount of total Fe³⁺. The difference between A₂ and A₁ (A₂ − A₁) is directly proportional to the TIBC concentration.
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