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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血清γ-谷氨酰转肽酶（γ-GT/GGT）主要来源于肝、胆系统。当肝胆发生疾病或损伤时，如阻塞性黄疸、胆汁性肝硬变、胆管炎、胆囊炎等，其活性显著升高；此外，饮酒、服药等亦可引起GGT活性升高。因此，GGT的测定对于肝胆疾病的临床诊断具有重要价值。 本试剂盒以L-γ-谷氨酰-3-羧基-4-苯基重氮酸为底物，双甘氨肽为谷氨酰基受体，在GGT催化下，底物上的谷氨酰基转移至双甘氨肽，同时生成呈色产物——5-氨基-2-硝基苯甲酸盐，该产物在405 nm处具有特征吸收峰。通过动力学模式连续监测单位时间内OD₄₀₅的初始线性增幅（ΔOD₄₀₅/min），即可精准计算样品中GGT的酶活力大小。
Serum γ‑glutamyltransferase (γ‑GT/GGT) is primarily derived from the liver and biliary system. Its activity is significantly elevated in hepatobiliary diseases or injuries, such as obstructive jaundice, biliary cirrhosis, cholangitis, and cholecystitis. In addition, alcohol consumption and certain medications can also increase GGT activity. Therefore, the determination of GGT is of great clinical value for the diagnosis of hepatobiliary diseases. This kit employs L‑γ‑glutamyl‑3‑carboxy‑4‑phenyldiazonic acid as the substrate and glycylglycine as the glutamyl acceptor. In the presence of GGT, the glutamyl group is transferred from the substrate to glycylglycine, simultaneously releasing a chromogenic product — 5‑amino‑2‑nitrobenzoate — which exhibits a characteristic absorption peak at 405 nm. By continuously monitoring the initial linear increase in absorbance at 405 nm per unit time (ΔOD₄₀₅/min) in kinetic mode, the GGT activity in the sample can be accurately calculated.
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