计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
3,5-二硝基水杨酸(DNS)是蔗糖合成酶SS-I(分解方向)活性检测试剂盒中的核心显色剂,其原理为:SS-I催化蔗糖和UDP生成果糖,果糖与DNS在加热条件下反应生成棕红色产物,该产物在540 nm处具有特征吸收峰,通过测定吸光度可定量果糖生成量,进而计算酶活性。
3,5‑Dinitrosalicylic acid (DNS) is the core chromogenic reagent in the assay kit for the cleavage direction activity of sucrose synthase (SS‑I). The principle is as follows: SS‑I catalyzes the reaction of sucrose and UDP to produce fructose. The generated fructose reacts with DNS under heating conditions to form a brown‑red product, which exhibits a characteristic absorption peak at 540 nm. By measuring the absorbance, the amount of fructose produced can be quantified, and the enzyme activity can be subsequently calculated.