计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖磷酸化酶(EC 2.4.1.7)是一种葡萄糖基转移酶,主要存在于微生物和植物中,催化合成葡萄糖基低聚糖,在食品、化妆品和医药领域应用广泛。本试剂盒采用NADPH偶联显色法测定其活力:SP催化蔗糖和磷酸生成1-磷酸葡萄糖,经偶联酶作用还原NADP⁺为NADPH,NADPH与显色剂反应生成450 nm处有最大吸收峰的黄色产物,通过检测其生成速率即可定量酶活性。
Sucrose phosphorylase (EC 2.4.1.7) is a glucosyltransferase primarily found in microorganisms and plants. It catalyzes the synthesis of glucosyl oligosaccharides and is widely used in the food, cosmetic, and pharmaceutical industries. This kit employs a NADPH‑coupled colorimetric method for the determination of its activity. The principle is as follows: SP catalyzes the reaction of sucrose and phosphate to produce glucose‑1‑phosphate. Through the action of coupling enzymes, glucose‑1‑phosphate leads to the reduction of NADP⁺ to NADPH. NADPH then reacts with a chromogenic reagent to produce a yellow product with a maximum absorption peak at 450 nm. The enzyme activity can be quantitatively determined by measuring the rate of formation of this product.