本产品为蔗糖水溶液，摩尔浓度精确配制为0.25 M。
在密度梯度离心应用中，本产品是细胞分离液进行亚细胞颗粒及病毒分离时的关键配套试剂。将细胞分离液原液与2.5 M蔗糖溶液（阿拉丁货号：S1527934）按9:1体积比混合制得等渗母液后，使用本产品将母液稀释至目标密度，用于线粒体、溶酶体、微粒体等细胞器及病毒、外泌体的等密度梯度离心分离。低浓度蔗糖体系可有效维持亚细胞组分的渗透压平衡，保护颗粒结构与功能完整性。此外，本产品还可用于渗透压调节、提取缓冲液配制、清洗液及试剂空白对照等常规生化实验。
使用说明
1. 无菌取用：实验前请检查瓶口封条是否完好。使用前请在超净工作台内通过移液枪或无菌量筒吸取所需体积，注意避免反复冻融和多次交叉取用。
2. 稀释等渗母液至目标工作液：根据实验所需密度，使用本产品将等渗母液（细胞分离液原液与2.5 M蔗糖溶液按9:1混合）稀释至目标工作液密度。稀释后的工作液建议即配即用，以避免长时间存放导致的污染或密度变化风险。具体体积计算公式可参考细胞分离液说明书。
3. 其他用途：如用于渗透压调节、提取缓冲液配制或清洗液等用途，请根据实验设计直接使用或进一步稀释，严格无菌操作，即配即用。
注意事项
1. 开瓶后应确保瓶盖拧紧，防止水分蒸发导致浓度改变或微生物污染。
2. 若长期保存后出现结晶析出（尤其低温环境下），请置于温水中轻微加热并充分摇匀至完全溶解后再使用；若发现溶液浑浊或有可见异物，请立即停止使用。
3. 本产品适用于亚细胞颗粒及病毒的分离体系。若分离细胞样品（如淋巴细胞、单核细胞），请使用NaCl体系（1.5 M NaCl溶液）。
4. 本品仅供实验室科研使用，严禁用于人体诊断或临床治疗。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
This product is an aqueous sucrose solution prepared to an accurate molar concentration of 0.25 M.
For density‑gradient centrifugation applications, it acts as an essential supporting reagent for isolating subcellular particles and viruses with cell separation medium. Prepare the isotonic stock master solution by mixing stock cell‑separation medium with 2.5 M Sucrose Solution (Aladdin Cat. No.: S1527934) at a 9:1 volume ratio, then dilute the master solution with this product to the target density for isopycnic density‑gradient centrifugation of organelles including mitochondria, lysosomes and microsomes, as well as viruses and exosomes. The low‑concentration sucrose system effectively maintains osmotic balance of subcellular components and preserves the structural and functional integrity of particles. Furthermore, this product can be applied to routine biochemical experiments for osmotic pressure adjustment, extraction‑buffer preparation, washing solution formulation and reagent blank control.
Instructions for Use
1.Sterile handling: Inspect the bottle seal for integrity before the experiment. Aspirate the required volume using a pipette or sterile graduated cylinder inside a biosafety cabinet. Avoid repeated freeze‑thaw cycles and repeated cross‑contaminating withdrawals.
2.Dilute isotonic stock master solution to target working solution: Dilute the isotonic stock master solution (prepared by mixing stock cell‑separation medium and 2.5 M sucrose solution at 9:1 ratio) with this product to the target working‑solution density according to experimental requirements. Prepare diluted working solutions fresh before use to prevent contamination or density drift caused by prolonged storage. Refer to the cell‑separation medium manual for detailed volume‑calculation formulas.
3.Other applications: For osmotic adjustment, extraction‑buffer preparation, washing solution and other purposes, use directly or further dilute following your experimental design. Perform strict aseptic manipulation and prepare fresh for immediate use.
Precautions
1.Tighten the cap firmly after opening to prevent concentration alteration due to water evaporation and microbial contamination.
2.If crystals precipitate after long‑term storage (especially under low‑temperature conditions), warm the bottle gently in lukewarm water and shake thoroughly until crystals are completely dissolved prior to use. Discontinue use immediately if turbidity or visible particulate matter is observed.
3.This product is intended for isolation systems of subcellular particles and viruses. For cell samples such as lymphocytes and monocytes, use NaCl‑based systems (1.5 M NaCl Solution).
4.For laboratory research use only. Not for human diagnostic or clinical therapeutic applications.
5.Wear a lab coat and disposable gloves for personal safety and health during operation.
ZJ26F1039682
危险品化学品经营许可证（带存储）