是否无菌：是
本产品为高浓度蔗糖水溶液，摩尔浓度精确配制为2.5 M。
在密度梯度离心应用中，本产品是细胞分离液进行亚细胞颗粒及病毒分离时的关键配套试剂。将细胞分离液原液与本产品按9:1体积比混合，可制得与细胞等渗的母液，再以0.25M蔗糖溶液稀释至目标密度后，用于线粒体、溶酶体、微粒体等细胞器及病毒、外泌体的等密度梯度离心分离。蔗糖体系可有效避免盐离子条件下亚细胞组分的聚集，保护颗粒结构与功能完整性。
此外，本产品还可作为冷冻保存保护剂或渗透压缓冲组分，用于维持生物样本在低温过程中的结构完整性，是实验室生化实验和组织培养的基础试剂。
使用说明
1. 无菌取用：实验前请检查瓶口封条是否完好。使用前请在超净工作台内通过移液枪或无菌量筒吸取所需体积，注意避免反复冻融和多次交叉取用。
2. 配制等渗母液（SIM）：将细胞分离液原液与本产品按9:1体积比混合（例如：90 mL分离液原液+10 mL 2.5M 蔗糖溶液）。建议将本产品缓慢加入分离液原液中，使用磁力搅拌器或涡旋振荡器确保混合均匀。
3. 稀释至目标工作液：根据实验所需密度，使用0.25 M蔗糖溶液将等渗母液稀释至目标工作液密度。稀释后的工作液建议即配即用，以避免长时间存放导致的污染或密度变化风险。具体体积计算公式可参考细胞分离液说明书。
4. 原位梯度分离：将待分离样品（亚细胞颗粒或病毒）与稀释后的工作液直接混合，随后进行高速离心，离心过程中自发形成连续密度梯度，目标颗粒迁移至等密度位置。
5. 其他用途：如用于渗透压调节或冷冻保存保护剂，请用无菌去离子水稀释至目标浓度并校准渗透压，严格无菌操作，即配即用。
注意事项
1. 开瓶后应确保瓶盖拧紧，防止水分蒸发导致浓度改变或微生物污染。
2. 若长期保存后出现结晶析出（尤其低温环境下），请置于温水中轻微加热并充分摇匀至完全溶解后再使用；若发现溶液浑浊或有可见异物，请立即停止使用。
3. 本产品适用于亚细胞颗粒或病毒的分离。若分离细胞样品（如淋巴细胞、单核细胞），请使用NaCl体系（1.5 M NaCl溶液）。
4. 本品仅供实验室科研使用，严禁用于人体诊断或临床治疗。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
This product is a high‑concentration aqueous sucrose solution formulated to an accurate molar concentration of 2.5 M.
For density‑gradient centrifugation applications, it serves as a critical supporting reagent for the isolation of subcellular particles and viruses using cell separation medium. Mix the stock cell‑separation medium with this product at a 9:1 volume ratio to prepare an isotonic stock master solution. Further dilute the master solution with 0.25 M sucrose solution to the target density for isopycnic density‑gradient centrifugation and separation of organelles including mitochondria, lysosomes and microsomes, as well as viruses and exosomes. The sucrose‑based system effectively prevents aggregation of subcellular components under salt‑ion conditions and preserves the structural and functional integrity of particles.
In addition, this product can be used as a cryoprotectant or osmotic buffer component to maintain the structural integrity of biological samples under low‑temperature conditions. It is a fundamental reagent for laboratory biochemical experiments and tissue culture.
Instructions for Use
1.Sterile handling: Inspect the bottle seal for integrity before use. Aspirate the required volume with a pipette or sterile graduated cylinder inside a biosafety cabinet. Avoid repeated freeze‑thaw cycles and repeated cross‑contaminating withdrawals.
2.Preparation of isotonic stock master solution (SIM): Mix the stock cell‑separation medium with this product at a 9:1 volume ratio (e.g., 90 mL stock cell‑separation medium + 10 mL 2.5 M sucrose solution). Slowly add this product into the stock cell‑separation medium, and mix thoroughly using a magnetic stirrer or vortex mixer.
3.Dilution to target working solution: Dilute the isotonic stock master solution with 0.25 M sucrose solution to the target working‑solution density according to experimental requirements. Prepare diluted working solutions fresh before use to prevent contamination or density drift upon prolonged storage. Refer to the cell‑separation medium manual for detailed volume‑calculation formulas.
4.In‑situ gradient separation: Directly mix the sample to be separated (subcellular particles or viruses) with the diluted working solution, then perform high‑speed centrifugation. A continuous density gradient forms spontaneously during centrifugation, and target particles migrate to their isopycnic positions.
5.Other applications: When used for osmotic adjustment or cryoprotection, dilute to the target concentration with sterile deionized water, calibrate osmotic pressure, perform strict aseptic manipulation, and prepare fresh for immediate use.
Precautions
1.Tighten the cap after opening to avoid concentration shift caused by water evaporation and microbial contamination.
2.If crystals precipitate after long‑term storage (especially under low‑temperature conditions), warm the bottle gently in lukewarm water and shake thoroughly until crystals are fully dissolved prior to use. Discontinue use immediately if turbidity or visible particulate matter is observed.
3.This product is intended for the isolation of subcellular particles and viruses. For cell samples such as lymphocytes and monocytes, use NaCl‑based systems (1.5 M NaCl Solution).
4.For laboratory research use only. Not for human diagnostic or clinical therapeutic applications.
5.Wear a lab coat and disposable gloves for personal safety and health during operation.
ZJ26F1039684
危险品化学品经营许可证（带存储）