本产品有效成分为棕榈酸钠（Sodium palmitate，10 mM）和油酸钠（Sodium oleate，20 mM）。本产品为无菌（0.22 μm过滤）溶液，可与完全培养基直接互溶用于实验。高脂细胞添加剂及溶剂对照均为淡黄色清亮液体，所用溶剂不含NaOH或有机溶剂，对细胞无毒性。本产品为组合装，是棕榈酸钠/油酸钠高脂复合添加剂的升级款（阿拉丁货号：S1527963），可直接用于诱导脂肪肝细胞模型。
产品组成
| 项目号 | 组件类型 | 组件名称-中文 | 组件名称-英文 | 物理外观 | 3×5 mL | 储存 | | S1527964A | 半成品 | 10 mM 棕榈酸钠BSA高脂细胞添加剂 | 10 mM Sodium palmitate-BSA high-fat cell culture additive | 液体 | 5 mL | -20°C | | S1527964B | 半成品 | 20 mM油酸钠BSA高脂细胞添加剂 | 20 mM Sodium oleate-BSA high-fat cell culture additive | 液体 | 5 mL | -20°C | | S1527964C | 半成品 | 溶剂对照 | Vehicle control | 液体 | 5 mL | -20°C |
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使用说明
1. 每次使用前置于室温或2~8℃冰箱过夜溶解，请勿直接加热溶解；如溶液不澄清，可在≤50℃水浴加热，且不能超过3 min。
2. 针对不同细胞种类及实验目的，棕榈酸钠、油酸钠所用浓度和比例不同，处理时间也不同，请根据实验需求进行预实验。
3. 对于首次使用本产品，建议采用浓度梯度处理细胞，摸索针对特定细胞、达到特定效果的适宜浓度。
4. 待细胞生长至 80-100%汇合度时，换成新鲜培养基（含血清），按适当浓度滴加棕榈酸钠/油酸钠，对照组加入等量体积（棕榈酸钠+油酸钠体积）溶剂对照，轻柔混匀后将细胞放入细胞培养箱中继续培养。
5. 加入本品24~48小时后，进行下一步分析。
注意事项
1. 收到产品后可分装，于-20℃长期保存；可在2~8℃短期保存。
2. 由于溶剂BSA影响吸光度，在使用CCK-8检测细胞毒性时，在弃掉高脂细胞添加剂与培养基混液后，需要用PBS清洗细胞2~3次，再加入CCK-8工作液。
The active ingredients of this product are sodium palmitate (10 mM) and sodium oleate (20 mM).It is a sterile solution (0.22 μm filtered), fully miscible with complete medium for experimental applications. Both the high‑fat cell additive and its vehicle control are pale‑yellow clear liquids. The solvent contains no NaOH or organic solvents and exhibits no cytotoxicity. Supplied as a combined kit, it is an upgraded version of Sodium palmitate/Sodium oleate high‑fat cell additive (Aladdin Cat. No.: S1527963), and can be directly used for inducing cellular fatty‑liver models.
Product Composition
| Component No. | Component Name | Appearance | 3×5 mL | Storage | | S1527964A | 10 mM Sodium palmitate-BSA high-fat cell culture additive | Liquid | 5 mL | -20°C | | S1527964B | 20 mM Sodium oleate-BSA high-fat cell culture additive | Liquid | 5 mL | -20°C | | S1527964C | Vehicle control | Liquid | 5 mL | -20°C |
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Instructions for Use
1.Thaw at room temperature or in a 2‑8 °C refrigerator overnight before each use. Do not heat directly. If the solution appears turbid, perform water‑bath heating at ≤50 °C for no more than 3 minutes.
2.The concentrations, ratio of sodium palmitate to sodium oleate, and treatment duration vary among different cell types and experimental objectives. Perform preliminary trials according to your experimental requirements.
3.For first‑time use, treat cells with a concentration gradient to identify the optimal concentration for your specific cell type and desired experimental outcome.
4.When cells reach 80‑100% confluence, replace with fresh serum‑supplemented medium. Add sodium palmitate / sodium oleate at the appropriate concentration. For the control group, add an equal volume of vehicle control (equal to the total volume of sodium palmitate plus sodium oleate). Mix gently and place the cells back into the cell‑culture incubator for further incubation.
5.Perform downstream analysis 24‑48 hours after product addition.
Precautions
1.Aliquot the product upon receipt. Store long‑term at −20 °C; short‑term storage is permitted at 2‑8 °C.
2.BSA in the solvent interferes with absorbance measurements. When assessing cytotoxicity using the CCK‑8 assay, discard the mixed solution of high‑fat cell additive and medium, wash the cells 2‑3 times with PBS, and then add CCK‑8 working solution.
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