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脱氧胆酸钠(Sodium deoxycholate,又称去氧胆酸钠),CAS:302-95-4,分子量 414.55,为常用生物化学试剂。本品为10%脱氧胆酸钠溶液,属于离子型去污剂,可有效裂解细胞、增溶难溶性蛋白质,广泛应用于蛋白质提取与细胞裂解实验;亦可用于微生物胆汁溶菌实验。胆盐凭借表面活性可激活细菌自溶酶,加速肺炎链球菌等菌体的自溶裂解,若开展胆汁溶菌鉴别实验,更推荐选用脱氧胆酸钠溶液 (10%, pH 7.0) 版本。 提示:本品为离子型表面活性剂,剧烈摇晃易产生大量持久泡沫;具有刺激性,操作时请佩戴手套,避免接触皮肤、眼睛及黏膜。 仅限科研使用,不可用于临床诊断。
Sodium deoxycholate (also known as sodium desoxycholate, CAS: 302-95-4, molecular weight: 414.55) is a commonly used biochemical reagent. This product is a 10% sodium deoxycholate solution, classified as an ionic detergent. It effectively lyses cells and solubilizes poorly soluble proteins, and is widely used in protein extraction and cell lysis procedures. It is also applied in the microbial bile solubility test. Bile salts, through their surfactant properties, activate bacterial autolytic enzymes, accelerating the autolysis of susceptible organisms such as Streptococcus pneumoniae. For bile solubility identification tests, the sodium deoxycholate solution (10%, pH 7.0) formulation is recommended. Note: This product is an ionic surfactant; vigorous shaking may generate persistent foaming. It is irritant; wear gloves during handling and avoid contact with skin, eyes, and mucous membranes. For research use only. Not for clinical diagnosis.