固定的目的在于保存细胞和组织的原有形态结构，固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长，固定剂通过凝固、生成添加化合物等使蛋白质内部结构发生改变，从而使酶失活，固定剂对细胞核细胞外成分发生物理改变。固定液主要分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等，较为常用的是醛类中的福尔马林、醇类中的乙醇；戊二醛固定液会引起蛋白质α-螺旋结构变形，不利于过氧化物酶染色。戊二醛固定液固定速度快，渗透力差。
本品为缓冲型复合固定液，主要由乙酸（醋酸）、乙酸钠（醋酸钠）、福尔马林（甲醛）及纯化水科学配比配制而成。体系为弱酸缓冲体系，pH稳定，兼顾固定穿透力与样本形态保护，配方成熟、重复性好，是寄生虫检测专用经典固定液。主要适用于各类肠道寄生虫样本的快速固定、长期保存、样本浓缩及后续永久染色制片。可有效固定寄生虫虫卵、幼虫、原虫等各类虫体结构，防止虫体自溶、变形、崩解与脱落，最大程度保留虫体完整形态与细微结构，适配粪便寄生虫检查、寄生虫标本制作、教学科研标本留存及实验室常规染色鉴定等场景。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）：
1.样本处理：新鲜粪便或寄生虫离体样本及时收集，尽量去除大块杂质，缩短样本离体暴露时间，避免虫体提前自溶。
2.固定浸泡：将待处理样本加入SAF固定液中，固定液用量需为样本体积的10-15倍，充分混匀，确保所有虫体、虫卵完全浸没在固定液中。
3.固定时间：常规寄生虫样本室温固定10-30min即可完成定型；用于长期保存的样本可直接浸泡留存，无需更换液体。
4.后续操作：固定完成后，可直接进行样本浓缩、沉淀、涂片制作，或进行苏木素伊红等永久染色、镜检观察，也可密封长期保存备用。
注意事项：
1.本品有一定腐蚀性，请在通风较好的环境下操作，避免吸入。
2.固定时保证样本完全浸没，避免局部暴露导致虫体自溶破损；不同样本分开处理，防止交叉污染。
3.本品仅用于实验室寄生虫样本固定与科研检测用，严禁用于人体、活体组织及临床治疗。
4.为了您的安全和健康，请穿实验服并戴一次性手套操作。
5.试剂开封后请尽快使用，以防影响后续实验效果。
The purpose of fixation is to preserve the original morphology and structure of cells and tissues. Fixatives prevent autolysis of tissues and cells by endogenous lysosomal enzymes, inhibit the growth of bacteria and molds, and inactivate enzymes by altering protein internal structures through coagulation, formation of additive compounds, and other mechanisms. Fixatives also induce physical changes in cellular and extracellular components. Fixatives are generally classified into aldehyde-based, mercury-based, alcohol-based, oxidant-based, and picrate-based types. The most commonly used are formaldehyde (from the aldehyde group) and ethanol (from the alcohol group). Glutaraldehyde fixatives can cause deformation of the protein α-helix structure, which is unfavorable for peroxidase staining. Glutaraldehyde fixatives exhibit rapid fixation but poor penetration.
This product is a buffered composite fixative, primarily formulated with acetic acid, sodium acetate, formaldehyde, and purified water in scientifically determined proportions. It features a weak acid buffer system with stable pH, balancing fixation penetration and sample morphology preservation. With a mature formulation and good reproducibility, it is a classic fixative specifically designed for parasitological testing. It is primarily suitable for rapid fixation, long-term preservation, sample concentration, and subsequent permanent staining and slide preparation of various intestinal parasite samples. It effectively preserves the structures of parasite eggs, larvae, protozoa, and other parasitic forms, preventing autolysis, deformation, disintegration, and detachment, while maximizing the retention of intact morphology and fine structural details. It is applicable to fecal parasite examinations, parasite specimen preparation, teaching and research specimen storage, and routine laboratory staining and identification.
This reagent is intended for research use only and is not suitable for clinical diagnosis or other applications.
Operating Instructions (For Reference Only):
1.Sample Preparation: Collect fresh fecal samples or isolated parasite specimens promptly. Remove large debris as much as possible and minimize the time of sample exposure ex vivo to prevent premature autolysis of parasites.
2.Fixation Immersion: Add the sample to be processed into the SAF fixative. The volume of fixative should be 10–15 times the sample volume. Mix thoroughly to ensure that all parasite eggs and organisms are completely submerged in the fixative.
3.Fixation Time: Routine parasite samples can be adequately fixed at room temperature within 10–30 minutes. For long-term preservation, samples may be directly left immersed in the fixative without the need for fluid replacement.
4.Subsequent Procedures: After fixation, samples can be directly subjected to concentration, sedimentation, smear preparation, or permanent staining (e.g., hematoxylin and eosin staining) and microscopic examination. Alternatively, they may be sealed and stored for long-term use.
Precautions:
1.This product is corrosive. Please operate in a well-ventilated area and avoid inhalation.
2.Ensure that samples are completely submerged during fixation to prevent localized exposure, which may lead to parasite autolysis and damage. Process different samples separately to avoid cross-contamination.
3.This product is intended for laboratory parasite sample fixation and research testing only. It is strictly prohibited for use on humans, living tissues, or clinical treatment.
4.For your safety and health, please wear a lab coat and disposable gloves during handling.
5.Please use the reagent promptly after opening to avoid compromising subsequent experimental results.
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