是否无菌:是
本产品用于冠状病毒、流感病毒、手足口病毒、麻疹风疹病毒等各种病毒相关唾液样本的采集、运输。能快速灭活病毒和酶的活性,含有特殊的核酸保护剂和稳定剂,含有保护液的病毒核酸在常温下可长期保存,半年内不降解。
本产品具有以下特点:
1. 含有高效酶灭活试剂,可以快速灭活包括DNase和RNase在内的各种酶,去除降解核酸的隐患。
2. 含病毒快速灭活试剂,可以在数分钟之内灭活各种DNA病毒和RNA病毒,使之失去传染性。本产品保存液中的病毒已经灭活,不能用于病毒培养。
3. 含有特殊的核酸保护剂和稳定剂,可以使释放出来的病毒DNA和病毒RNA在常温下长期保存无明显降解。
4. 保存的样品可以在常温运输。
5. 保存的样品可以直接用于后续的核酸纯化 (需要自备核酸纯化试剂盒),所得核酸可用于PCR等后续扩增实验。
使用方法:
注意:尽管本试保存液含有病毒灭活剂,但不能排除离心管外在取样时污染了病毒,因此操作人员必须按处理传染性样品的方法进行相应的防护,必须在合乎国家规定的实验室进行所有操作。
1. 采样前,在采样管的标签上标注相关样品信息,例如姓名,采样日期等。
2. 将唾液样本加入采样管中,加入等量的唾液病毒保存液。一般收集2ml唾液,也可根据需要的量收集。
3. 盖上管盖,上下颠倒混匀。
4. 核酸纯化和检测:用相关唾液核酸纯化试剂盒纯化核酸并进行检测,未用完的样品短期室温放置,如需要长期保存可于-20°C~-70°C保存。
注意:由于病毒有传染性,因此实验过程中的所有垃圾需要按有传染性物质处理。操作人员也必须做好相应防护。
Is it sterile: Yes
This product is designed for the collection and transportation of saliva samples associated with various viruses, including coronavirus, influenza virus, hand‑foot‑and‑mouth virus, measles virus, rubella virus, etc. It rapidly inactivates viruses and enzymatic activity, and contains proprietary nucleic‑acid protective agents and stabilizers. Viral nucleic acids in the preservation solution can be stored long‑term at ambient temperature without degradation for up to six months.
This product features the following advantages:
1. Contains high‑efficiency enzyme‑inactivating reagents that rapidly inactivate various enzymes including DNase and RNase, eliminating risks of nucleic‑acid degradation.
2. Contains rapid virus‑inactivation reagents that render diverse DNA and RNA viruses non‑infectious within minutes. Viruses in the preservation solution are fully inactivated; this product shall not be used for virus culture.
3. Formulated with proprietary nucleic‑acid protective agents and stabilizers, enabling released viral DNA and RNA to be stored long‑term at ambient temperature with no substantial degradation.
4. Preserved samples are transportable under ambient‑temperature conditions.
5. Preserved samples can be directly subjected to subsequent nucleic‑acid purification (nucleic‑acid purification kit required separately). Purified nucleic acids are compatible with downstream amplification assays such as PCR.
Instructions for Use:
Note: Although the preservation solution contains virus‑inactivating agents, potential viral contamination on the exterior of the centrifuge tube during sampling cannot be ruled out. Operators must implement appropriate personal protective measures as required for handling infectious specimens. All operations shall be performed in laboratories complying with national regulatory requirements.
1. Before sample collection, mark relevant sample information (e.g., name, sampling date) on the label of the sampling tube.
2. Add saliva sample into the sampling tube, followed by an equal volume of saliva virus preservation solution. Typically, collect 2 mL of saliva; sample volume may be adjusted according to experimental requirements.
3. Secure the tube cap and mix thoroughly by inverting the tube up and down.
4. Nucleic‑acid purification and detection: Purify nucleic acids using a compatible saliva nucleic‑acid purification kit prior to detection. Unused samples may be stored briefly at room temperature. For long‑term storage, keep samples at ‑20 °C to ‑70 °C.
Note: Given the infectious nature of viruses, all experimental waste must be disposed of as infectious material. Operators shall maintain adequate personal protection throughout the whole procedure.