计算溶液所需的质量、体积或浓度。
BioReagent,适用于免疫组织化学(for IHC) 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
平衡盐溶液(Balanced Salt Solution, BSS)的 pH、渗透压与细胞生长生理环境相近,能够维持体系渗透压与酸碱平衡,提供细胞存活、代谢所需的无机盐组分,部分配方可提供能量物质,满足体外实验条件下细胞存活与基础代谢需求。其主要成分为无机离子,配方中可添加碳酸氢钠、葡萄糖、酚红;必要时可加入 HEPES 稳定 pH。平衡盐溶液可作为完全培养基的基础液,也可用于稀释浓缩氨基酸、维生素溶液,用于配制完全培养基。
Sorensen磷酸缓冲液 (10×, pH 6.2) 主要由磷酸氢二钠、磷酸二氢钾、氯化镁、氯化钙配制而成,磷酸根终浓度 170 mM,多用于免疫组化、组织切片及其他常规实验。 本试剂仅用于科研领域,不适用于临床诊断或其他用途。
组分与存储
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操作步骤(仅供参考):
临用前取50 mL磷酸缓冲液加入适量去离子水中,再加入0.1 mL钙盐溶液,补去离子水至500 mL,调pH值至6.2,即为Sorensen磷酸缓冲液 (1×, pH6.2)。
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。
2、试剂开封后请尽快使用,以防影响后续实验效果。
Balanced Salt Solution (BSS) matches the pH and osmotic pressure of the physiological environment for cell culture. It maintains osmotic pressure and acid-base balance, supplies inorganic components required for cell survival and metabolism to support cell viability and basal metabolism in in vitro experiments. It consists mainly of inorganic ions; formulations may contain sodium bicarbonate, glucose and phenol red. HEPES can be supplemented when needed for pH stabilization. BSS can serve as the basal solution for complete culture medium, or be used to dilute concentrated amino acid and vitamin solutions for medium preparation.
Sorensen Phosphate Buffer (10×, pH 6.2) is composed of disodium hydrogen phosphate, potassium dihydrogen phosphate, magnesium chloride and calcium chloride, with a final phosphate concentration of 170 mM. It is widely used for immunohistochemistry, tissue section preparation and other general laboratory applications.
For research use only. Not for clinical diagnosis or other applications.
Components & Storage
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Procedure (For reference only):
Before use, add 50 mL phosphate buffer into an appropriate volume of deionized water, then add 0.1 mL calcium salt solution. Top up with deionized water to 500 mL and adjust pH to 6.2 to obtain 1× Sorensen's Phosphate Buffer (pH6.2).
Precautions:
1. For your safety and health, wear a lab coat and disposable gloves during operation.
2. Use the reagent promptly after opening to avoid compromising subsequent experimental results.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for Immunohistochemistry(IHC) 级别指南 →