计算溶液所需的质量、体积或浓度。
痰消化液 (Sputasol法)
有货
为什么选择此级别
BioReagent,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
痰液、体液、粪便等为微生物学检验常用标本，含有多种微生物且基质成分复杂。结核分枝杆菌营养要求高、生长缓慢；培养时标本内杂菌增殖快，易消耗营养，干扰结核分枝杆菌检出。因此，含杂菌标本（如痰标本、消化道标本、支气管肺泡灌洗液、经支气管吸出液等）在培养前需进行前处理，用于标本液化与杂菌杀灭；尿液、尸检组织及其他受污染液体标本，培养前需做去污处理。血、脑脊液、关节液等取自机体无菌部位、无杂菌污染的标本，可直接离心，取沉淀接种。
常用标本前处理消化试剂包括硫酸、氢氧化钠、N‑乙酰‑L‑半胱氨酸 (NALC)、苯扎溴铵、草酸、胰酶、二硫苏糖醇 (DTT) 等。痰消化液（Sputasol 法）主要由 DTT 与磷酸盐组成，经无菌处理，可直接用于痰液标本均质化操作。
自备材料：
1、痰液、体液、粪便等样本。
2、生理盐水、PBS、培养基。
3、接种环、离心管、离心机、剪刀、研钵。
操作步骤 (仅供参考)：
(一) 痰液样本
1、挑起约 5ml 浓痰液至已标记的 50ml 离心管中。
2、加入等量的痰消化液进行均质化处理，涡旋震荡 20s，30～37℃ 静置 15～20min。
3、加入无菌 PBS (pH6.8 左右) 至约 50ml，3000r/min 离心 15min。
4、倒掉上清液，添加 PBS 1~3ml。
(二) 体液样本
1、无菌体液直接接种，取 10ml 体液于离心管中离心，取沉淀物直接接种。
2、污染的体液操作同痰液标本。
(三) 组织样本
1、取 1g 组织用剪刀剪碎加入 5ml 培养基，用研钵将组织制作成匀浆，加入 50ml 离心管。
2、以下操作同痰液标本。
(四) 粪便样本
1、取 1g 粪便加入到 50ml 离心管中，并向其中加入 5ml 培养基，混匀。
2、以下操作同痰液标本。
注意事项：
1、痰液样本宜在清晨采集，采集前先以冷开水充分漱口，以免口腔正常菌群污染样本。
2、尿液样本宜收集早晨第一次尿的中段尿为宜。
3、样本采集后应立即送检。
4、如样本用于结核杆菌的检查，痰量要多。
5、肺部感染的样本可能患有菌血症，应同时做血液培养。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Specimens such as sputum, body fluids and feces are commonly used in microbiological tests, which contain diverse microorganisms and complex endogenous components. Mycobacterium tuberculosis has high nutritional requirements and slow growth. During culture, contaminants in specimens grow rapidly and consume nutrients, interfering with the detection of Mycobacterium tuberculosis. Therefore, specimens containing contaminants (e.g., sputum specimens, gastrointestinal specimens, bronchoalveolar lavage fluid, transbronchial aspirates) require pre-treatment prior to culture for specimen liquefaction and decontamination. Urine, autopsy tissues and other contaminated liquid specimens also need decontamination before culture. Specimens collected from sterile sites of the body without contamination, such as blood, cerebrospinal fluid and synovial fluid, can be directly centrifuged, and the sediment is used for inoculation.
Common digestion reagents for specimen pre-treatment include sulfuric acid, sodium hydroxide, N-acetyl-L-cysteine (NALC), benzalkonium bromide, oxalic acid, trypsin, dithiothreitol (DTT), etc. Sputasol (sputum digestion solution) mainly consists of DTT and phosphate salts, sterile-processed, and can be directly applied for homogenization of sputum specimens. For research use only. Not for clinical diagnosis or other applications.
Required Materials:
1. Sputum, body fluid, stool and other samples
2. Normal saline, PBS, culture medium
3. Inoculating loop, centrifuge tubes, centrifuge, scissors, mortar
Operating Procedures (For Reference Only):
(I) Sputum Specimens
1. Transfer approximately 5 mL of thick sputum into a labeled 50 mL centrifuge tube.
2. Add an equal volume of sputum digestion solution for homogenization. Vortex for 20 seconds, then incubate at 30–37 °C for 15–20 minutes.
3. Add sterile PBS (pH~6.8) up to 50 mL, centrifuge at 3000 r/min for 15 minutes.
4. Discard the supernatant, then resuspend the pellet in 1–3 mL of PBS.
(II) Body Fluid Specimens
1. For sterile body fluids: directly take 10 mL fluid into a centrifuge tube for centrifugation, and inoculate the sediment.
2. For contaminated body fluids: process following the protocol for sputum specimens.
(III) Tissue Specimens
1. Take 1 g tissue, cut into small pieces with scissors, add 5 mL culture medium, homogenize using a mortar, then transfer the homogenate into a 50 mL centrifuge tube.
2. Follow the subsequent steps of the sputum specimen protocol.
(IV) Fecal Specimens
1. Add 1 g feces into a 50 mL centrifuge tube, mix with 5 mL culture medium thoroughly.
2. Follow the subsequent steps of the sputum specimen protocol.
Precautions:
1. Sputum samples are preferably collected in the early morning. The subject should rinse the mouth thoroughly with cool boiled water before sampling to avoid contamination by normal oral flora.
2. For urine samples, the midstream portion of the first morning urine is recommended.
3. Samples should be delivered to the laboratory immediately after collection.
4. If the sample is used for the detection of Mycobacterium tuberculosis, an adequate volume of sputum is required.
5. Patients with pulmonary infection may be complicated with bacteremia; blood culture should be performed simultaneously.
6. Use the reagent promptly after opening the package to avoid compromised experimental results.
规格
别名
Sputasol 痰消化液
英文别名
ADS088DZ0 | BB-111480 | DZ0801 | G4700 | S771868 | R30144 | BL2950A
规格或纯度
BioReagent, 无菌
稳定性与储存
Store at -20℃ long term (12 months).
英文名称
Sputum Digestion Solution (Sputasol Method)
储存条件
-20°C储存
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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