计算溶液所需的质量、体积或浓度。
BioReagent,无菌,适用于免疫组织化学(for IHC) 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
柠檬酸钠又称枸橼酸钠,在工业上可以作为螯合剂,在医药行业可作为抗凝剂,在生物学上柠檬酸钠多与柠檬酸配合作为柠檬酸盐使用。柠檬酸钠缓冲液在免疫组化过程中可以有效去除醛类固定试剂导致的蛋白之间的交联,充分暴露石蜡切片等样品中的抗原表位,从而大大改善免疫染色效果。
柠檬酸钠缓冲液 (0.1mol/L,pH4.5,无菌)是柠檬酸与柠檬酸钠按照不同比例混合而得,其柠檬酸根浓度为0.1mol/L ,pH值约为4.5,经无菌处理,常用于溶解链脲佐菌素(Streptozotocin,STZ),可用于糖尿病动物模型造模。
该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
根据实验具体要求操作。
注意事项:
1、密闭保存,避免pH发生变化。
2、由于该试剂含有柠檬酸盐,保质期较短,易产生絮状物,建议尽快使用。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Sodium citrate, also known as sodium citrate tribasic, serves as a chelating agent in industry and an anticoagulant in the pharmaceutical sector. In biological applications, it is frequently combined with citric acid to form citrate buffers. During immunohistochemistry, sodium citrate buffer effectively eliminates protein‑protein cross‑links induced by aldehyde fixatives and fully exposes antigenic epitopes in paraffin‑embedded sections, thereby greatly improving immunostaining performance.
Sodium Citrate Buffer (0.1 mol/L, pH 4.5, sterile) is prepared by mixing citric acid and sodium citrate at appropriate ratios. It features a citrate concentration of 0.1 mol/L with an approximate pH of 4.5 and is sterile‑filtered. This buffer is commonly used for dissolving Streptozotocin (STZ) in the establishment of diabetic animal models.
For research‑only use. Not intended for clinical diagnosis or other applications.
Procedure (for reference only):
Follow the specific requirements of your experiment.
Precautions:
1. Store in a tightly‑sealed container to prevent pH drift.
2. Containing citrate salts, this reagent has a relatively short shelf‑life and may form flocculent precipitates. Prompt use is recommended.
3. Use promptly after opening to avoid compromised experimental results.
| pH | 4.5 |
|---|
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Sterile 级别指南 → 查看 Suitable for Immunohistochemistry(IHC) 级别指南 →