计算溶液所需的质量、体积或浓度。
概述
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
规格
规格或纯度
MW 30000 Da
英文名称
Succinimidyl valeric acid PEG, mPEG-SVA
生化机理
一般来说，NHS PEG 摩尔过量 10 到 50 倍于含胺材料的量就能充分缀合：PEG NHS 储存液：50 mg 加入 1 mL 结合缓冲液：反应步骤：将目标材料溶解在连接缓冲液中。估计目标材料上伯胺基团的浓度。向目标连接材料中加入 NHS PEG 原液，最终浓度至少保持在 10 mg/mL。最佳连接需要 10~50 摩尔过量的 PEG NHS；让混合物在室温下搅拌 30~60 分钟或在 4 0C 下搅拌 2 小时。注：此处显示的结构主要代表来自 PEG 的官能团，可能无法显示确切的连接体。如果您对连接体有任何疑问，请联系我们。
储存条件
-20°C储存
运输条件
超低温运输
名称和识别符
分子类型
聚合物
高级数据
化学和物理性质
溶解性
Off-white/white solid or viscous liquid depends on molecule weight;10 mg/mL, clear in water, DMSO, Chloroform;
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器
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