计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Description
This plasmid is a perfect substrate for assaying any topoisomerase. pHOT-1 is relatively small (<3KB) which is ideal for displaying intermediate topoisomers. Since all topoisomerases display degenerate DNA binding and cleavage sequences, pHOT-1 will effectively support activity for a wide array of topoisomerase. Supercoiled pHOT-1 is a scaled-down derivative of pBR322 and optimized for higher yields. It has one additional feature for researchers who wish to assess human topo I cleavage at a single sequence: we have included the human topoisomerase I preferential binding site (the hexadecameric sequence described by Westergaard and co-workers, see Bonven et al., 1985) that is derived from the tetrahymena ribosomal gene repeat. This construct is useful as a topo I cleavage substrate since the enzyme efficiently cleaves at the hexadecameric site in the absence of camptothecin. This allows the investigator to rigorously evaluate new potential topo I inhibitors because a single background cleavage is a built-in control; if the agent stimulates cleavage at this and/or any other sequence in the fragment that contains the hexadecameric site, prominent cutting is readily revealed. Typically, it is difficult to trap topo I cleavages on DNA; one must use more enzyme since cleavage complexes consume the enzyme stoichiometrically. For this reason, higher levels of enzyme are required for analysis of DNA cleavages. The fragment can be isolated from the polylinker using the sites shown below (protocols in Maniatis cloning book), end labeled and used as a cleavage substrate.
Shipping&storage
Supercoiled pHOT-1 DNA is shipped at ambient temperature and should be stored at 4°C.
规格
别名
超螺旋 pHOT-1 DNA
英文名称
Supercoiled pHOT-1 DNA
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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