Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.Streptolysin O is a toxin secreted byStreptococcus pyogenesand is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.Permeabilizes membranes to permit cellular uptake of large or charged molecules.
Preparation instructions
Form
Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.
Principle
规格
规格或纯度
lyophilized powder,Protein ~3 % by Lowry,25,000-50,000 U/vial,来源于化脓链球菌
英文名称
Streptolysin O
单位定义
一单位将导致2%红细胞悬液在磷酸缓冲液(pH 7.4)中37 °C孵育30 min后发生50%溶解
生化机理
可透过动物细胞膜、由巯基活化的毒素。该蛋白作为单体与膜胆固醇结合,随后聚合成 >12 nm 孔隙周围的大弧形和环形结构。Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic.