计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Product Characteristics
The Synthetic Blocking Buffer, Blotting is a readyto-use solution for blocking of the remaining free binding sites after coating or transfer of biomolecules to a membrane. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal.
The Synthetic Blocking Buffer, Blotting eliminates the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA
Composition & Properties
The Synthetic Blocking Buffer, Blotting is a readyto-use buffer. The buffer contains no proteins, and cross reactivity is therefore avoided.
Working Procedure
1.Follow the normal procedure for transfer of the biomolecules to the membrane.
2.Dry the membrane.
3.Block the membrane by incubation in the Synthetic Blocking Buffer, Blotting for 5-10 minutes at room temperature and gentle shaking.
4.Wash the membrane in a standard washing buffer for blotting.
5. Continue the processing as usual.
Tips & Tricks
• In ELISA, the Synthetic Blocking Buffer, ELISA (cat. no. S494401) is recommended.
Handling & Storage
• Store solution at 2-8 °C.