Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
规格
产品名称
Texas red标记的链霉亲和素
别名
链霉亲和素，Texas Red™ 结合物 | 重组链霉亲和素 | 链霉亲和素
英文别名
SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
Recombinant Streptavidin Protein (Texas red) (S639037) – IF/ICC IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (Texas red) (S639037) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (Texas red) (S639037) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jarkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Texas red) (S639037) at 1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
IF/ICC
1-10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Flow Cytometry
1.0 -10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
ZJ25F0825438
危险品化学品经营许可证（带存储）