计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
SID 3712249 (MiR-544 Inhibitor 1) is a miR-544 biogenesis inhibitor. SID 3712249 binds directly to the precursor miRNA. SID 3712249 blocks production of the mature microRNA and decreases miR-544, HIF-1α, and ATM transcripts. SID 3712249 can be used in the research of cancers, such as breast cancer
In Vitro
SID 3712249 (compound 1, 20 nM, 48 h) disrupts miR-544-mediated inhibition of BMI1 based on significant increases in the RFP/BFP ratio. SID 3712249 (20 nM, 48 h) inhibits precursor but not mature miR-544 binding to BMI1 or mTOR3’-UTR. SID 3712249 (20 nM, 48 h) results in accumulation of pre-miR-544 and a decrease in miR-544 levels in MCF10A cells. SID 3712249 (20 nM, 5 days) induces apoptosis in MDA-MB-231 and MCF- 7 cells and has no effect on cell survival in normoxic conditions. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Apoptosis AnalysisCell Line: MDA-MB-231, MCF-7 cells Concentration: 20 nM Incubation Time: 5 days Result: Induced apoptosis and has no effect on cell survival in normoxic conditions (flow cytometric analysis of Annexin V and PI stainning).
In Vivo
SID 3712249 (compound 1, 100 μL of 40 μM, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model . SID 3712249 (20 nM, pre-treated GFP-labeled MDA-MB-231 cells, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: MDA-MB-231-GFP-luc tumor model Dosage: 100 μL of 40 μM Administration: Intraperitoneal injection Result: Inhibited tumor growth (evidenced by live animal bioluminescent imaging) with no overt side effects. Decreased levels of miR-544, ATM, and HIF-1α and increased levels of mTOR (resected tumor samples).
IC50& Target:MiR-544
规格
规格或纯度
10mM in DMSO
英文名称
SID 3712249
生化机理
SID 3712249（MiR-544 抑制剂 1）是一种 miR-544 生物发生抑制剂。SID 3712249 可直接与 miRNA 前体结合。SID 3712249 可阻止成熟 microRNA 的产生，并减少 miR-544、HIF-1α 和 ATM 的转录本。SID 3712249 可用于
储存条件
-80℃储存
运输条件
特低温运输
名称和识别符
EC号
100-822-0
分子类型
小分子
Isomeric SMILES
C1CCN(C1)C2=NC(=C3C(=C2)C(=C(N=C3N)N4CCCC4)C#N)N
PubChem CID
分子量
323.4
技术文档
高级数据
关联靶点（人）
APAF1
Tchem
凋亡蛋白酶活化因子 1(Apoptotic protease-activating factor 1)
(1 活性数据)
安全和危险性（GHS）
RIDADR
NONHforallmodesoftransport
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）