计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品为由Super Pfx DNA Polymerase、Mg2+、dNTPs以及反应缓冲液组成的优化的2×预混型 试剂，只需要添加引物和DNA模板即可进行PCR反应。Super Pfx DNA Polymerase具有3´→5´外 切酶活性，其保真性约为Taq DNA聚合酶的100倍，是克隆的理想选择。该酶通过引入延伸增 强技术，在维持高保真性的同时，实现了延伸速度10sec/kb的高速PCR，扩增长度可达20kb。 此外，本产品在兼具高成功率的同时，对于扩增反应困难的长片段、GC-rich区域和过量模板 也可以进行扩增。扩增得到的PCR产物的3´端不带有“A”碱基，可直接克隆于平末端载体中。 本产品分为不含Loading Dye的标准型2×Master Mix和含有Loading Dye 的2×Master Mix。
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保存条件：-20℃保存。
产品内容
产品特征
1. 可进行高速PCR
扩增1kb以下的目的片段时，延伸时间可设为10sec。扩增1-10kb的目的片段时，延伸时间可 设为20-30sec/kb。相比以往试剂可大幅度缩短PCR的反应时间。
2. 高保真性
保真性约为Taq DNA polymerase的100倍，长目的片段也可以迅速且高保真性地扩增，扩增产 物可用于多种用途。
3. 简便
本试剂中包含所有除引物、模板以外的其他PCR组分，便于操作的同时也提高了实现结果的重 现性。另外，2×Super Pfx Master Mix（Dye）中含有 Loading Dye，反应 结束后，可直接点样进行凝胶电泳。
注意事项
带有尿嘧啶的引物和模板不适用于本产品。
使用说明
PCR反应体系
1. 反应液制备前，请将试剂完全融解、混匀后再使用。
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・组分添加完后请混合均匀并迅速转移到PCR仪里。
・引物终浓度推荐为0.4-0.5μM，但扩增10kb以上的长片段时，引物终浓度为0.3μM可提高扩增产物量。
PCR反应程序
1. 反应液制备前，请将试剂完全融解、混匀后再使用。
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注意：
1）预变性：对于多数纯化后的模板，98℃，30sec即可；对于复杂模板可延长预变性时间，不超3min。
2）退火：一般实验中退火温度比引物Tm低3-5℃，发生非特异性反应时，适当提高退火温度。如果需要， 可以建立一个温度梯度寻找引物与模板结合的最适温度。对于高Tm的引物，可以使用两步循环，将退火和 延伸合并为一步。
3）延伸：对于复杂的基因组样本，延伸时间通常为20-30sec/kb，但对于简单模板(质粒、大肠杆菌等)或
＜1kb的复杂模板，延伸时间可缩短至10sec/kb。如果需要，cDNA或＞6kb的复杂模板，延伸时间可以增
加到40-50sec/kb。
Products content
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Products Introduction
It is an optimized 2× premixed reagent consisting of Super Pfx DNA Polymerase, Mg2+, dNTPs, and reaction buffer, and requires only the addition of primers and DNA templates for the PCR reaction.Super Pfx DNA Polymerase has a 3´→5´ exonuclease activity, and is approximately 100 times more fidelity than Taq DNA Polymerase. Super Pfx DNA Polymerase has 3´→5´ exonuclease activity and is about 100 times more fidelity than Taq DNA Polymerase, making it ideal for cloning. With the introduction of elongation enhancement technology, this enzyme enables high-speed PCR with an elongation speed of 10 sec/kb and an amplification length of up to 20 kb, while maintaining high fidelity. In addition, this product combines a high success rate with the ability to amplify long fragments that are difficult to amplify, GC-rich regions, and templates that are too large to be amplified. The amplified PCR product does not have an "A" base at the 3´ end and can be directly cloned into a flat-end vector. This product is divided into the standard 2×Master Mix without Loading Dye and the 2×Master Mix with Loading Dye.
Product Characteristics
1. High-speed PCR possible
The extension time can be set to 10sec for amplification of target fragments of less than 1kb, and 20-30sec/kb for amplification of target fragments of 1-10kb, which significantly reduces the PCR reaction time compared to conventional reagents.
2. High fidelity
The fidelity of Taq DNA polymerase is about 100 times higher than that of Taq DNA polymerase, and long target fragments can be amplified quickly and with high fidelity, and the amplified products can be used for a variety of purposes.
3. simple and convenient
This reagent contains all PCR components except primers and templates, which facilitates the operation and improves the reproducibility of the results. In addition, 2×Super Pfx Master Mix (Dye) contains Loading Dye, which can be used for gel electrophoresis directly after the reaction.
caveat
Primers and templates with uracil are not suitable for this product.
Instructions for use
PCR Reactors
1. Before preparing the reaction solution, please melt and mix the reagents completely before use.
After adding the components, please mix them well and transfer them to the PCR instrument quickly.
・The recommended final primer concentration is 0.4-0.5 μM, but for amplification of long fragments of 10 kb or more, a final primer concentration of 0.3 μM can increase the amount of amplified product.
PCR reaction program
1. Before preparing the reaction solution, please melt and mix the reagents completely before use.
take note of
1）Pre-denaturation: for most purified templates, 98℃, 30sec is sufficient; for complex templates, the pre-denaturation time can be extended, not more than 3min.
2）Annealing: In general, the annealing temperature is 3-5°C lower than the primer Tm. When a non-specific reaction occurs, increase the annealing temperature appropriately. If necessary, a temperature gradient can be established to find the optimal temperature for primer-template binding. For high Tm primers, a two-step cycle can be used, combining annealing and extension into one step.
3）Extension: for complex genomic samples, extension times are typically 20-30sec/kb, but can be reduced to 10sec/kb for simple templates (plasmids, E. coli, etc.) or complex templates <1kb. cDNAs, or complex templates >6kb, can be increased to 40-50sec/kb, if desired.
规格
别名
2×Super Pfx 预混液
英文名称
2×Super Pfx Master Mix
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
biological_products
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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