计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
|
产品简介
本品为由Super Kfx DNA Polymerase、Mg2+ 、dNTPs以及PCR稳定剂和增强剂组 成的预混体系，浓度为2×。Super Kfx DNA Polymerase是一种快速、高扩增效率的高 保真DNA聚合酶，该酶具有5′-3′DNA聚合酶活性和3′-5′外切酶活性，具有扩增能力 强，保真度高，特异性强等优点。2×Mix中添加了独特的扩增增强因子及延伸因子，独 特的配方使整个反应体系非常稳定，操作方便，适用于各种片段及模板的扩增。本产 品适用于基因克隆、二代建库扩增、基因定点突变、SNP等扩增实验。
质量控制
经检验无外源核酸酶活性，能有效地扩增各种模板；2-8℃存放一个月，无明显活 性改变。
使用方法
以下举例为常规PCR反应体系和反应条件，实际操作中应根据模板、引物结构和目的片
段大小不同进行相应的改进和优化。 PCR 反应体系
所有操作请在冰上进行，各组分解冻后请充分混匀，用完之后请及时放回-20℃保存。
1. PCR 反应体系
所有操作请在冰上进行，各组分解冻后请充分混匀，用完之后请及时放回-20℃保存。
|
2. PCR 反应条件
|
注意：
1）优先使用三步法扩增，三步法无法扩增目的产物或引物Tm值大于68°C，请尝试两步法。
2）变性：简单模板的预变性98℃，30s-1min，对于复杂的模板，预变性时间可延长至3min。
3）退火：一般实验中退火温度比引物的Tm值低3-5℃，如无法得到理想的扩增效率时，应梯度改变退 火温度，进行优化；发生非特异性反应时，适当提高退火温度。
4）延伸：延伸时间应根据所扩增片段的长度和模板复杂程度设定，本产品扩增效率为4-6kb/min， 对于长片段及复杂性高的模板建议2-4kb/min。
5）循环次数：可根据扩增产物的下游应用设定循环数，如果循环次数太少，扩增量不足，循环次数太 多，错配机率会增加，所以在保证产物得率的前提下应尽量减少循环次数
Products content
|
Products Introduction
This product is a premixed system consisting of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers at a concentration of 2×. Super Kfx DNA Polymerase is a fast, high-fidelity DNA polymerase with high amplification efficiency, which possesses 5′-3′DNA polymerase activity and 3′-5′ exonuclease activity. With 5′-3′ DNA polymerase activity and 3′-5′ exonuclease activity, the enzyme has the advantages of strong amplification ability, high fidelity and high specificity, etc. 2×Mix has added unique amplification enhancement factors and extension factors, and the unique formula makes the whole reaction system very stable and easy to operate, which is suitable for the amplification of various fragments and templates. The product is suitable for gene cloning, second generation library amplification, targeted gene mutation, SNP amplification experiments.
quality control
No exogenous nuclease activity, can effectively amplify various templates; stored at 2-8℃ for one month, no obvious activity change.
Usage
The following examples are conventional PCR reaction systems and conditions, which should be improved and optimized according to the template, primer structure and target fragment size.
1. PCR reaction system
All operations should be carried out on ice, please mix the components thoroughly after thawing, after use, please promptly return to -20 ℃ for storage.
2. PCR reaction system
take note of
1）Priority is given to three-step amplification; if the three-step method fails to amplify the target product or if the primer Tm value is greater than 68°C, try the two-step method.
2）Denaturation: pre-denaturation of simple templates 98°C, 30s-1min, for complex templates, the pre-denaturation time can be extended to 3min.
3）Annealing: In general, the annealing temperature is 3-5℃ lower than the Tm value of the primers. If the desired amplification efficiency cannot be obtained, the annealing temperature should be changed in a gradient to optimize the results; if a non-specific reaction occurs, the annealing temperature should be increased appropriately.
4）Extension: The extension time should be set according to the length of the amplified fragments and the complexity of the template, the amplification efficiency of this product is 4-6kb/min, for long fragments and templates with high complexity it is recommended that 2-4kb/min.
5）Cycling times: the number of cycles can be set according to the downstream application of the amplified product. If the number of cycles is too small, the amplification will be insufficient, and if the number of cycles is too large, the chance of mismatch will be increased, so the number of cycles should be minimized under the premise of guaranteeing the yield of the product.
规格
英文名称
2×Super Kfx MasterMix
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）