25-35循环计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
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产品简介
本品为由Super Kfx DNA Polymerase、Mg2+ 、dNTPs以及PCR稳定剂和增强剂组 成的预混体系，浓度为2×。Super Kfx DNA Polymerase是一种快速、高扩增效率的高 保真DNA聚合酶，该酶具有5′-3′DNA聚合酶活性和3′-5′外切酶活性，具有扩增能力 强，保真度高，特异性强等优点。2×Mix中添加了独特的扩增增强因子及延伸因子，独 特的配方使整个反应体系非常稳定，操作方便，适用于各种片段及模板的扩增。本产 品适用于基因克隆、二代建库扩增、基因定点突变、SNP等扩增实验。本产品已加入 染料（蓝色），反应结束后可直接进行琼脂糖电泳检测，操作方便简单。
质量控制
经检验无外源核酸酶活性，无宿主残留DNA，能有效地扩增各种模板。
使用方法
以下举例为常规PCR反应体系和反应条件，实际操作中应根据模板、引物结构和目的 片段大小不同进行相应的改进和优化。
1. PCR 反应体系
所有操作请在冰上进行，各组分解冻后请充分混匀，用完之后请及时放回-20℃ 保存。
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2. PCR 反应条件
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注意：
1）优先使用三步法扩增，三步法无法扩增目的产物或引物Tm值大于68°C，请尝试两步法。
2）变性：简单模板的预变性98℃，30s-1min，对于复杂的模板，预变性时间可延长至3min。
3）退火：一般实验中退火温度比引物的Tm值低3-5℃，如无法得到理想的扩增效率时，应梯度改变 退火温度，进行优化；发生非特异性反应时，适当提高退火温度。
4）延伸：延伸时间应根据所扩增片段的长度和模板复杂程度设定，本产品扩增效率为4-6 kb/min， 对于长片段及复杂性高的模板建议2-4 kb/min。
5）循环次数：可根据扩增产物的下游应用设定循环数，如果循环次数太少，扩增量不足，循环次数
太多，错配机率会增加，所以在保证产物得率的前提下应尽量减少循环次数。
Product content
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Product Introduction
This product is a premixed system composed of Super Kfx DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2 ×. Super Kfx DNA Polymerase is a fast and highly efficient high fidelity DNA polymerase with 5 ′ -3 ′ DNA polymerase activity and 3 ′ -5 ′ exonuclease activity. It has advantages such as strong amplification ability, high fidelity, and strong specificity. The addition of unique amplification enhancers and elongation factors in 2xMix results in a unique formula that makes the entire reaction system very stable, easy to operate, and suitable for amplification of various fragments and templates. This product is suitable for gene cloning, second-generation library amplification, gene directed mutagenesis, SNP amplification experiments, etc. This product has been added with dye (blue), and after the reaction is completed, agarose electrophoresis detection can be directly performed, making the operation convenient and simple.
Quality control
After testing, there is no exogenous nuclease activity and no residual host DNA, which can effectively amplify various templates.
Usage
The following are examples of conventional PCR reaction systems and reaction conditions. In practical operation, corresponding improvements and optimizations should be made based on different templates, primer structures, and target fragment sizes.
1. PCR reaction system
All operations should be carried out on ice. After each group is decomposed and frozen, please mix thoroughly. After use, please put it back at -20 ℃ for storage in a timely manner.
2. PCR reaction conditions
Attention:
1) Priority should be given to using the three-step method for amplification. If the three-step method cannot amplify the target product or the Tm value of the primer is greater than 68 ° C, please try the two-step method.
2) Denaturation: Pre denaturation of simple templates at 98 ℃ for 30 seconds to 1 minute. For complex templates, the pre denaturation time can be extended to 3 minutes.
3) Annealing: In general experiments, the annealing temperature is 3-5 ℃ lower than the Tm value of the primer. If the ideal amplification efficiency cannot be achieved, the annealing temperature should be gradually changed for optimization; When non-specific reactions occur, increase the annealing temperature appropriately.
4) Extension: The extension time should be set based on the length of the amplified fragment and the complexity of the template. The amplification efficiency of this product is 4-6 kb/min. For long fragments and high complexity templates, it is recommended to set 2-4 kb/min.
5) Cycle count: The number of cycles can be set based on the downstream application of the amplification product. If there are too few cycles, insufficient amplification, or too many cycles, the probability of mismatch will increase. Therefore, while ensuring product yield, the number of cycles should be minimized as much as possible.
规格
别名
高保真酶预混液 | 2×Super Kfx MasterMix(染料)
英文名称
2×Super Kfx MasterMix (Dye)
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
biological_products
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品应如何储存？
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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