计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
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产品简介
SuperStain是一种生物大分子，不能穿透细胞膜进入细胞内。相对于EB的强诱变性，是一种安全无毒的核酸染料。SuperStain与EB具有相同的光谱特性，无需改变滤光片及观察装置，在正常紫外光激发检测即可，集无毒性、高灵敏性和高稳定性等优点于一体，可以选择胶染法或泡染法进行染色，使用非常方便和灵活。
自备试剂
琼脂糖
TAE/TBE电泳缓冲液
实验前准备及重要注意事项
1．使用泡染法染色时，染料用量较多，单次使用的染色液可重复使用3次左右。3×染色液可以大量制备，在室温下避光保存。
2．如果总是看到条带弥散或分离不理想，建议使用泡染法染色以确认问题是否与染料有关。如果染色后问题依旧存在，则说明问题与染料无关，请尝试：选择合适的琼脂糖浓度；选用更长的凝胶；延长凝胶时间以保证边缘清晰；减少DNA上样量（推荐上样量为50-200ng/泳道）。
操作步骤
1．胶染法向琼脂糖溶液中加入SuperStain(10,000×in Water)至终浓度为1×（如琼脂糖溶液为50ml，则加入5μl染料），并充分混匀。待凝胶凝固后，按照常规方法进行电泳和图像采集。
注意：由于SuperStain具有良好热稳定性，可以直接添加到热的琼脂糖溶液中而不需要等待溶液冷却。摇晃，振荡或者翻转以保证染料充分混匀。也可以选择将SuperStain加到琼脂糖粉末和电泳缓冲液中，然后用微波炉或其他常用方式加热以制备琼脂糖凝胶。
2．泡染法
1）配置不含染料的琼脂糖凝胶，按照常规方法进行电泳。
2）用H2O将SuperStain(10,000×in Water)稀释约3,300倍到0.1M NaCl中，制成3×染色液，如将15μl SuperStain (10,000×in Water) 和5ml 1M NaCl加到45ml H2O中。
3）将凝胶小心地放入合适的容器中，缓慢加入足量的3×染色液浸没凝胶。室温振荡染色30min左右，最佳染色时间根据凝胶厚度以及琼脂糖浓度不同而略有不同。按照常规方法进行图像采集。
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图为Super DNA Marker在含有SuperStain染料的琼脂糖凝胶中的电泳图，从左到右主带上样量为(150ng，75ng)。
Product content
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Product Introduction
SuperStain is a biological macromolecule that cannot penetrate cell membranes to enter cells. Compared with the strong mutagenicity of EB, it is a safe and non-toxic nucleic acid dye.SuperStain has the same spectral characteristics with EB, no need to change the filter and observation device, it can be detected by normal UV excitation, combining the advantages of non-toxicity, high sensitivity and high stability, and it can be stained by gel staining or bubble staining, which makes it very convenient and flexible to use.
Self-contained reagents
agarose
TAE/TBE Electrophoresis Buffer
Pre-experiment Preparation and Important Notes
When dyeing with the soak dyeing method, the amount of dye is high, and a single use of the dye solution can be reused about 3 times.3 x Dye solution can be prepared in large quantities and stored at room temperature, protected from light.
If you consistently see dispersed or poorly separated bands, it is recommended that you use a bubble stain to confirm that the problem is not dye related. If the problem persists after staining, the problem is not dye-related, so try: choosing the right agarose concentration; choosing a longer gel; prolonging the gel time to ensure that the edges are clear; and decreasing the amount of DNA sampled (the recommended amount is 50-200ng/lane).
Operation steps
1. Gel dyeing method
Add SuperStain (10,000×in Water) to the agarose solution to a final concentration of 1× (5 μl of dye if the agarose solution is 50 ml) and mix thoroughly. After the gel has solidified, electrophoresis and image acquisition are performed in the usual way. Note: Due to its good thermal stability, SuperStain can be added directly to hot agarose solution without waiting for the solution to cool. Shake, oscillate or turn to ensure the dye is well mixed. Optionally, SuperStain can be added to agarose powder and electrophoresis buffer and then heated in a microwave or other commonly used means to prepare an agarose gel.
2. Dyeing method
1) Configure a dye-free agarose gel and perform electrophoresis according to conventional methods.
2) Dilute SuperStain (10,000×in Water) about 3,300 times into 0.1 M NaCl with H2O to make a 3× staining solution, e.g., add 15 μl SuperStain (10,000×in Water) and 5 ml of 1 M NaCl to 45 ml of H2O.
3) Carefully place the gel into a suitable container and slowly add sufficient amount of 3× staining solution to submerge the gel. Oscillate the staining at room temperature for about 30 min. The optimal staining time varies slightly depending on the thickness of the gel as well as the concentration of agarose. Image acquisition was performed according to conventional methods.
The graph on the left shows the electrophoresis of Super DNA Marker in an agarose gel containing SuperStain dye, with sample volumes on the main strip from left to right (150ng, 75ng).
规格
别名
超级染色剂
规格或纯度
10,000× in Water
英文名称
SuperStain
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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