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产品内容
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产品简介
本产品在常规6×Loading Buffer中添加了SuperStain染料,该染料是一种生物大分子, 不能穿透细胞膜进入细胞内。相对于EB的强诱变性,是一种安全无毒的核酸染料。使用6×SuperStain Loading Buffer替代原始Loading Buffer时,在制胶过程中无需加入EB等核酸染料,只需要将DNA与6×SuperStain Loading Buffer混匀即可进行凝胶电泳。由于SuperStain与EB具有相同的光谱特性,无需改变滤光片及观察装置,在正常紫外光激发检测即可,使用非常方便和灵活。
自备试剂
琼脂糖
TAE/TBE电泳缓冲液
实验前准备及重要注意事项
1.由于该染料只需在电泳前与DNA混匀,无需加入凝胶中,可使制备的凝胶保存时间更长。2.由于Loading Buffer中染料的量远高于将染料加入凝胶中的量,对DNA的灵敏度更高。
操作步骤
1.配置合适浓度的琼脂糖凝胶
2.吸取5 μl DNA样品与1 μl 6×SuperStain Loading Buffer混匀后,直接电泳于上样孔内,
进行常规电泳和图像采集。
Product content
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Product Introduction
This product adds SuperStain dye to the conventional 6×Loading Buffer, which is a biomacromolecule that cannot penetrate the cell membrane to enter the cell. It is a safe and non-toxic nucleic acid dye compared to the strong mutagenicity of EB. When using 6×SuperStain Loading Buffer instead of the original Loading Buffer, there is no need to add nucleic acid dyes such as EB during the gel preparation process, and all that is needed is to mix the DNA with 6×SuperStain Loading Buffer for gel electrophoresis. Since SuperStain and EB have the same spectral characteristics, there is no need to change the filter and observation device, and it can be detected under normal UV excitation, which is very convenient and flexible.
Self-contained reagents
agarose
TAE/TBE Electrophoresis Buffer
Pre-experiment Preparation and Important Notes
1. Since the dye only needs to be mixed with the DNA prior to electrophoresis and does not need to be added to the gel, the prepared gel can be preserved for a longer period of time.
2. Because the amount of dye in the Loading Buffer is much higher than the amount of dye added to the gel, it is more sensitive to DNA.
Operation steps
1. Configuration of agarose gels of appropriate concentration
2. Pipette 5μl of DNA sample and mix with 1μl of 6×SuperStain Loading Buffer, then directly electrophoresis in the sample wells for routine electrophoresis and image acquisition.