计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
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产品简介
SuperPro Multiplex PCR Mix是一款适用于各种类型多重PCR的预混体系，浓度为 2.5×，包含DNA聚合酶、PCR Buffer、dNTPs、Mg²+以及稳定剂和增强剂等成分，本 品只需要加入引物和模板即可进行扩增，操作简单方便，减少了污染几率，提高了检 测通量和重现性。
SuperPro Multiplex PCR Mix包含的DNA聚合酶是一种经基因工程改造的重组酶， 具有5’→3’DNA聚合酶活性，无5’→3’外切酶活性；DNA聚合酶经过新型抗体修饰，为 抗体修饰热启动酶，能够有效减少在常温条件下由引物和模板非特异性结合或引物二 聚体而产生的非特异性扩增，同时具有激活时间短、扩增能力强、灵敏度高、稳定性 好等优良特点。独特的PCR缓冲体系与热启动酶的组合，显著提高了PCR的扩增效率， 灵敏度更高，抑制物耐受性更强。
SuperPro Multiplex PCR Mix应用范围广，适用于各种类型多重PCR，如微卫星分
析、扩增子建库、基因分型以及SNP检测等。
注意事项
1.使用前请在本品完全融化后上下颠倒轻轻混匀，并经短暂离心后使用。
2.避免反复冻融本品，反复冻融可能使产品性能下降。本品可置于-20℃长期保存
使用方法
1. PCR反应体系
提取DNA扩增反应体系：
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注意： 引物设计时，应尽量减小各引物的Tm间的差值，差值尽量控制在5℃以内。各引物浓度请以终浓度 0.05-0.2 μM作为设定范围的参考。扩增效率不高的情况下，可提高引物浓度；发
2. PCR反应程序
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注意：
1) 一般实验中退火温度比扩增引物的熔解温度Tm低5℃，无法得到理想的扩增效率时，适当降低退火 温度;发生非特异性反应时，提高退火温度，由此优化反应条件。
2) 可根据扩增产物的下游应用设定循环数。如果循环次数太少，扩增量不足;如果循环次数太多，错配 机率会增加，非特异性背景严重。所以在保证产物得率的前提下应尽量减少循环次数。
3) PCR产物极易产生气溶胶污染，进而导致实验结果不准确、可信度不高等问题。建议将PCR反应体系
配制区和PCR反应区进行物理隔离，使用专用的移液器等设备，并定时对各实验区域进行清洁（使用
0.5%次氯酸钠或10%漂白剂进行擦拭清理)，以保证实验结果的可信度。
Products content
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Products Introduction
SuperPro Multiplex PCR Mix is a pre-mixed system for all types of multiplex PCRs at 2.5× concentration, containing DNA polymerase, PCR Buffer, dNTPs, Mg²+ as well as stabilizers and enhancers, etc. The product is easy to use and convenient to operate by adding primers and templates, which reduces contamination and improves the throughput and reproducibility of the assay. It reduces the chance of contamination and improves the detection throughput and reproducibility.
The DNA polymerase contained in the SuperPro Multiplex PCR Mix is a genetically engineered recombinant enzyme with 5'→3' DNA polymerase activity and no 5'→3' exonuclease activity. The DNA polymerase has been modified by a new type of antibody and is an antibody-modified hot start enzyme, which can effectively reduce the non-specific amplification caused by the non-specific binding of primers and templates or primer dimers at room temperature, and at the same time, it has the excellent features of short activation time, strong amplification ability, high sensitivity, good stability, etc. The unique PCR buffer system and hot start enzyme can be used for the production of the DNA polymerase. The unique combination of PCR buffer system and hot starter enzyme significantly improves PCR amplification efficiency, sensitivity and inhibitor tolerance.
SuperPro Multiplex PCR Mix has a wide range of applications and is suitable for all types of multiplex PCR, such as microsatellite analysis, amplicon banking, genotyping and SNP detection.
caveat
1. Before use, please mix the product gently by turning it up and down after it has been completely melted and centrifuged for a short period of time.
2. Avoid repeated freezing and thawing of this product, repeated freezing and thawing may degrade the performance of the product. This product can be stored at -20℃ for a long time.
Usage
1. PCR reaction system
Extract the DNA amplification reaction system:
Note: When designing the primers, the difference between the Tm of each primer should be minimized, and the difference should be controlled within 5℃ as much as possible. Please use the final concentration of 0.05-0.2 μM as a reference for setting the range of each primer concentration. If the amplification efficiency is not high, the primer concentration can be increased; if non-specific amplification occurs, the primer concentration can be decreased to optimize the reaction system. For optimal amplification, it is recommended that the primer mixture be vortexed for 10 s and centrifuged briefly before use.
2. PCR reaction program
Attention:
1) In general, the annealing temperature is 5℃ lower than the melting temperature of the amplification primer, Tm. When the desired amplification efficiency cannot be obtained, the annealing temperature should be lowered appropriately; when a non-specific reaction occurs, the annealing temperature should be increased, thus optimizing the reaction conditions.
2) The number of cycles can be set according to the downstream application of the amplified product. If the number of cycles is too low, the amplification amount will be insufficient; if the number of cycles is too high, the chance of mismatch will increase and the non-specific background will be serious. Therefore, the number of cycles should be minimized under the premise of ensuring the product yield.
3) PCR products are easily contaminated by aerosols, which leads to inaccurate and unreliable results. It is recommended to physically separate the PCR preparation area from the PCR reaction area, use special pipettes and other equipment, and clean the experimental areas regularly (using 0.5% sodium hypochlorite or 10% bleach to wipe and clean) to ensure the credibility of the experimental results.
规格
别名
SuperPro 多重 PCR 混合物
规格或纯度
1ml
英文名称
SuperPro Multiplex PCR Mix
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
biological_products
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器
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