计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
概述
胶原纤维 (Collagen Fiber) 是结缔组织中分布最广含量最多的一种纤维，广泛分布于各种脏器，其中皮肤、巩膜、肌腱最丰富。Ⅰ型胶原纤维主要是骨、皮肤、肌腱纤维；Ⅱ型 胶原纤维主要是软骨胶原；Ⅲ型胶原纤维主要在胚胎组织、成人血管、胃肠道；Ⅳ型胶原纤维主要在基膜中。天狼星红与其衬染液都是强酸性染料，易与胶原分子中的碱性基团结合，吸附牢固。偏振光镜检查，胶原纤维有正的单轴双折射光的属性，与天狼星红复合染色液结合后，可增强双折射，提高分辨率，从而区分Ⅰ型和Ⅲ型胶原纤维。
本产品由天狼星红染色液及分化液组成，主要用于各种组织病变时对胶原纤维异常或纤维增生的研究，在普通光学显微镜下心脏血管等组织的胶原纤维被染成红色，在偏振光镜下对各种纤维化病变的分型和分级研究有一定的帮助作用，采用免疫组化技术也可显示Ⅰ型和Ⅲ型胶原纤维，但所用抗体昂贵，操作费时，而采用天狼星红染色试剂便宜，操作简单。
| S774173 | Component | 3×250 mL | Storage |
| S774173A | 天狼星红染色液 | 250 mL | 2-8℃. Store in the dark |
| S774173B | 分化液 | 2×250 mL | 2-8℃. Store in the dark |
使用说明：
1、取材组织块，经组织固定液固定后，常规石蜡包埋，切片。
2.、切片用二甲苯脱蜡，经各级乙醇至水洗：二甲苯 (Ⅰ) 5min→二甲苯 (Ⅱ) 5min→100％乙醇 2min→95％乙醇 1min→80％乙醇 1min→75％乙醇 1min→蒸馏水洗 2min。
3、将切片完全浸入天狼星红染色液中染色10-60 min (根据上色情况调整染色时间)。
4、分化液快速冲洗2次，一次5s。
5、自来水浸泡5-15 min。
6、常规脱水，透明，封片：95％乙醇 (Ⅰ) 1min→95％乙醇 (Ⅱ) 1min→100％乙醇 (Ⅰ) 1min→100％乙醇 (Ⅱ) 1min→二甲苯 (Ⅰ) 1min→二甲苯 (Ⅱ) 1min→中性树脂封固，镜下观察。
7、染色结果：
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注意事项：
1、为使在偏振光镜下显示清晰，本法的切片厚度以6-7 μm为宜。
2、胞核也可以用Mayer苏木素染色液复染，它不影响Ⅰ型和Ⅲ型胶原纤维的数量和双折射强度的显示；也可采用其他明矾苏木素，但应缩短染色时间，否则容易过染。 3、本产品染色稳定，不易褪色。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、该试剂仅用于科研领域，不宜用于临床诊断或其他用途。
Collagen Fiber is the most widely distributed and abundant fiber in connective tissue, found extensively in various organs, with the highest concentrations in the skin, sclera, and tendons. Type I collagen fibers are primarily found in bone, skin, and tendon fibers; Type II collagen fibers are mainly cartilage collagen; Type III collagen fibers are primarily present in embryonic tissue, adult blood vessels, and the gastrointestinal tract; Type IV collagen fibers are mainly found in basement membranes. Sirius Red and its counterstain are both strong acid dyes that easily bind to basic groups in collagen molecules, forming a firm bond. Under polarized light microscopy, collagen fibers possess the property of positive uniaxial birefringence. When combined with Sirius Red complex staining solution, birefringence is enhanced, and resolution is improved, allowing for the differentiation of Type I and Type III collagen fibers.
This product consists of Sirius Red staining solution and a differentiation solution. It is mainly used for the study of collagen fiber abnormalities or fibrosis in various tissue pathologies. Under a regular light microscope, collagen fibers in tissues such as the heart and blood vessels are stained red. Under polarized light microscopy, it aids in the classification and grading of various fibrotic lesions. Immunohistochemical techniques can also be used to demonstrate Type I and Type III collagen fibers, but the required antibodies are expensive and the procedure is time-consuming. In contrast, using the Sirius Red stain is cost-effective and simple to perform.
| S774173 | Component | 3×250 mL | Storage |
| S774173A | Sirius Red Staining Solution | 250 mL | 2-8℃. Store in the dark |
| S774173B | Differentiation Solution | 2×250 mL | 2-8℃. Store in the dark |
Instructions
Collect tissue blocks, fix them in a tissue fixative, and perform routine paraffin embedding and sectioning.
Deparaffinize sections with xylene, hydrate through graded alcohols to water: Xylene (I) 5 min → Xylene (II) 5 min → 100% ethanol 2 min → 95% ethanol 1 min → 80% ethanol 1 min → 75% ethanol 1 min → Distilled water wash 2 min.
Completely immerse the sections in Sirius Red Staining Solution for 10-60 min (adjust staining time based on coloring intensity).
Rinse quickly with Differentiation Solution twice, 5 seconds each time.
Soak in tap water for 5-15 min.
Perform routine dehydration, clearing, and mounting: 95% ethanol (I) 1 min → 95% ethanol (II) 1 min → 100% ethanol (I) 1 min → 100% ethanol (II) 1 min → Xylene (I) 1 min → Xylene (II) 1 min → Mount with neutral resin and observe under a microscope.
Staining Results
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Precautions
For optimal visualization under polarized light microscopy, a section thickness of 6-7 μm is recommended for this method.
Nuclei can be counterstained with Mayer's hematoxylin staining solution; it does not affect the visualization or birefringence intensity of Type I and Type III collagen fibers. Other alum hematoxylins can also be used, but the staining time should be shortened to avoid overstaining.
This product provides stable staining that is not prone to fading.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This reagent is intended for research use only and is not suitable for clinical diagnosis or other applications.
规格
规格或纯度
BioReagent, 用于显微镜, 生物染色剂
稳定性与储存
Store at 2-8°C long term (12 months). Store in the dark.
英文名称
Picro Sirius Red Stain Kit
储存条件
2-8°C储存,避光
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F1039865 |
分析证书 | S774173 |
| ZJ26F0535212 |
分析证书 | S774173 |
| ZJ26F0434104 |
分析证书 | S774173 |
| ZJ25F0320004 |
分析证书 | S774173 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Histological and Cytological Section Staining: Principles and Methodological Essentials for Routine Pathology Stains and Structural Special Stains
目录： 技术文章
Applications and Comparison of Histological Counterstaining, Collagen Evaluation, and Protein Staining Methods
目录： 技术文章
Staining-Based Differentiation of Collagen Fibers, Muscle Fibers, and Fibrin in Tissue Sections
目录： 技术文章
此产品的引用文献
溶液计算器
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