TP0480066 is a selective topoisomerase II inhibitor with IC 50 s of 1.10 and 62.89 nM for DNA gyrase and topo IV , respectively. TP0480066 shows good activity of againsting various bacterial species including drug-resistant strains. TP0480066 also exhibits potent inhibitory activity to N. gonorrhoeae , can be used in study of gonorrhea.