Topoisomerase I/II inhibitor 3 (compound 7) is a potent topoisomerase I ( Topo I ) and II ( Topo II ) dual inhibitor. Topoisomerase I/II inhibitor 3 can inhibit cell proliferation, invasion and migration, and induce apoptosis by inhibiting PI3K /Akt/mTOR signaling pathway. Topoisomerase I/II inhibitor 3 can be used for liver cancer research.