Tubulin polymerization-IN-68 (compound 32) is a tubulin inhibitor that can inhibit tubulin polymerization and destroy the cellular microtubule network. Tubulin polymerization-IN-68 can upregulate the expression of PARP-1 and caspase-3 and induce cell apoptosis , and has anticancer activity. Tubulin polymerization-IN-68 can effectively inhibit HepG2 ( IC 50 =93 nM) and significantly inhibit the growth of HepG2 xenograft tumors in nude mice by oral administration.