T.cruzi-IN-4 (Compound 21) is an anti-parasitic agent. T.cruzi-IN-4 inhibits the trypomastigote and amastigote form of Trypanosoma cruzi with IC 50 of 0.033 μM (120 h), T.cruzi-IN-4 inhibits the promastigote and amastigote form of Leishmania infantum with IC 50 of 28.74 and 61.82 μM. T.cruzi-IN-4 inhibits Leishmania amazonensis promastigote form with IC 50 of 18.38 μM.