TRK II-IN-1 is a potent type II TRK inhibitor, with IC 50 s of 3.3, 6.4, 4.3 and 9.4 nM, for TRKA/B/C and TRKA G667C , respectively. TRK II-IN-1 also inhibits FLT3 , RET , and VEGFR2 with IC 50 s of 1.3, 9.9, and 71.1 nM, respectively. TRK II-IN-1 can be used for the research of TRK driven cancers.