TRK/ALK-IN-1 (compound 21) is a potent and dual inhibitor of TRK and ALK. TRK/ALK-IN-1 in the enzymatic assays is in good accordance with anti-proliferative activity with IC 50 values of 2.2, 9.3 and 38 nM towards TRKA , ALK WT and ALK L1196M , respectively. TRK/ALK-IN-1 has the potential for the research of cancer diseases.