计算溶液所需的质量、体积或浓度。
对甲苯磺酰基磁珠
只有 %1 左
概述
磁微粒化学发光免疫分析是将磁性分离技术、化学发光技术、免疫分析技术三者结合的分析方法。磁微粒作为载体偶联抗体或抗原，可捕获目标分子，因其具有超顺磁性可实现多次磁分离，有利于检测仪器的全自动化并加快检测速度。目前，磁微粒化学免疫分析技术因其灵敏度高、检测范围宽、自动化程度高等优点已被广泛应用于生物医学检测领域。
对甲苯磺酰基磁珠表面有对甲苯磺酰基修饰，可与蛋白共价结合，表面具有丰富的活性基团，无需进一 步活化，即可在温和碱性条件下直接用于与多种生物配基 （抗体、活性蛋白和多肽等）的氨基或巯基的共价偶联，形成的亚胺结构稳定，不易降解；同时，对甲苯磺酰基磁珠兼具偶联工艺易操作，易放大的特点，是生物大分子固定化的理想选择。
| 参数 | T1506717-1.2μm | T1506717-2.8μm |
| 外观 | 红棕色悬浊液 | 红棕色悬浊液 |
| 固含量 | 10 mg/mL | 10 mg/mL |
| 粒径及分布 | 1.2± 0.1 μm, CV＜5% | 2.8± 0.1 μm, CV＜5% |
| 表面基团含量 | 40-80 μmol/g | 40-90 μmol/g |
产品特点
1、弥散式结构：磁核材料为超顺磁性γ-Fe₂O₃纳米颗粒
2、氧化铁含量：12%-20%（不同磁珠有差异）
3、粒径：常规1.2、2.8μm
4、 粒径高度均一，批间差CV＜5%：保证稳定可靠的性能
5、分散性优良，检测本底低：自主创新设计的独特包覆层结构，具有极低的非特异性吸附
6、单批次2公斤级产能：满足化学发光客户日常需求
7、高稳定性：长时间存储后不会出现氧化变色和核磁泄露现象
使用说明
1、偶联条件选择
(1) 偶联缓冲液不应含有伯胺基团，例如 Tris 缓冲液、甘氨酸缓冲液等。
(2) 推荐偶联缓冲液为硼酸盐缓冲液（pH 9.5），此外，建议可尝试 pH 7.0（PBS）~9.5（硼酸盐），以筛选最佳偶联条件。
(3) 以IgG抗体为例，推荐偶联比例为 IgG:Beads=20~30μg:1mg；但由于偶联蛋白的多样性，建议筛选不同的偶联比例，以获得最优结果。
(4) 推荐偶联在37℃条件下进行，时长建议12h~18h。
(5) 偶联完成需要进行封闭，封闭蛋白类型、用量建议进行筛选（常用BSA、酪蛋白等），推荐在37℃条件下封闭6~16h。
2、使用注意事项
(1) 磁性微球在取用前，务必充分振荡使磁珠呈均匀的悬浮状态。
(2) 干燥、冷冻操作极易引起磁珠团聚，且会影响磁珠表面功能基团的活性，请注意保存条件。
3、偶联推荐方案
| 名称 | 详述 | 配制方法简述 |
| 偶联缓冲液A | 0.1M 硼酸盐缓冲液, pH 9.5 | 0.618g H₃BO₃ (硼酸, MW 61.83)，使用80mL纯化水溶解，用5M NaOH调节pH到9.5，补加纯化水至100mL |
| 偶联缓冲液B | 含3.0M硫酸铵的缓冲液A, pH 9.5 | 39.64g (NH₄)₂SO₄ (硫酸铵，MW 132.14)，使用80mL偶联缓冲液A溶解，用5M NaOH调节pH到9.5，补加偶联缓冲液A至100mL |
| 封闭液 | 50mM Tris+1.0% BSA+0.1% Tween 20, pH 7.4 | 0.606g Tris (三羟甲基氨基甲烷, MW 121.14)，1.0g BSA，0.1g Tween20使用80mL纯化水溶解，调节pH到7.4，补加纯化水至100mL |
| 保存液 | 50mM Tris+1.0% BSA+0.1% Tween 20, pH 7.4 | 同上 |
4、偶联方法
(1) 使用涡旋混匀器将对甲苯磺酰基磁珠充分混匀悬浮，取10mg到离心管中；
(2) 将离心管放置在磁力架上1~2min，移除上清液；
(3) 加入1.0 mL的偶联缓冲液A，涡旋分散磁珠后，按照步骤 (2) 移除上清；
(4) 重复 (3) 步骤2次；
(5) 加入200μg抗体，用偶联缓冲液A补充至总体积为0.6mL，涡旋分散磁珠；
(6) 加入0.4mL偶联缓冲液B，涡旋混匀；
(7) 保持磁珠旋转混匀，在 37℃条件下16h后按照步骤 (2) 移除上清；
(8) 加入1.0mL封闭液，涡旋分散磁珠，保持磁珠旋转混匀，在37°C条件下6~16h，按照步骤 (2) 移除上清；
(9) 加入1.0mL保存液，涡旋分散磁珠后，按照步骤 (2) 移除上清；
(10) 重复步骤 (9) 2次；
(11) 加入1.0mL磁珠保存液，涡旋混匀，得到浓度为10mg/mL的磁珠混悬液，2~8℃下保存。
应用实例
1、T1506717-1.2μm在抗穆勒氏管激素（AMH）检测中与进口品牌D发光值对比
结论：
相同的磁珠偶联、试剂检测条件下，
T1506717-1.2μm磁珠在AMH检测中发光值高于进口品牌D同类型1.0μm磁性微球；
T1506717-1.2μm三批发光值差异极小，生产工艺稳定批间差小。
2、T1506717-2.8μm在癌胚抗原（CEA）检测中与进口品牌D发光值对比
结论：
相同的磁珠偶联、试剂检测条件下，
T1506717-2.8μm磁珠在CEA检测中发光值高于进口品牌D同类型2.8μm磁性微球；
T1506717-2.8μm三批发光值差异极小，生产工艺稳定批间差小。
Magnetic Particle Chemiluminescence Immunoassay is an analytical method that combines magnetic separation technology, chemiluminescence technology, and immunoassay technology. Magnetic particles, as carriers conjugated with antibodies or antigens, can capture target molecules. Due to their superparamagnetic properties, they enable multiple magnetic separations, facilitating full automation of detection instruments and accelerating testing speed. Currently, magnetic particle chemiluminescence immunoassay technology is widely used in biomedical detection due to its high sensitivity, broad detection range, and high automation.
Tosyl Magnetic Beads are surface-modified with tosyl groups, enabling covalent binding to proteins. The surface possesses abundant active groups, allowing direct covalent conjugation with various biological ligands (such as antibodies, active proteins, and peptides) via their amino or thiol groups under mild alkaline conditions, without requiring further activation. The resulting imine structure is stable and resistant to degradation. Additionally, tosyl magnetic beads feature an easy-to-operate and easily scalable conjugation process, making them an ideal choice for the immobilization of biomacromolecules.
| Parameter | T1506717-1.2μm | T1506717-2.8μm |
| Appearance | Red-brown suspension | Red-brown suspension |
| Solid Content | 10 mg/mL | 10 mg/mL |
| Particle Size & Distribution | 1.15± 0.1 μm, CV＜5% | 2.8± 0.1 μm, CV＜5% |
| Surface Group Content | 40-80 μmol/g | 40-90 μmol/g |
Product Features
Diffuse Structure: The magnetic core consists of superparamagnetic γ-Fe₂O₃ nanoparticles.
Iron Oxide Content: 12% - 20% (varies depending on the specific bead type).
Particle Size: Standard sizes of 1.2 μm and 2.8 μm.
Highly Uniform Particle Size with CV < 5% between Batches: Ensures stable and reliable performance.
Excellent Dispersion and Low Detection Background: Features a uniquely designed, proprietary coating layer that exhibits extremely low non-specific adsorption.
Production Capacity of 2 kg per Batch: Meets the routine demands of chemiluminescence customers.
High Stability: No oxidation/discoloration or magnetic core leakage occurs after long-term storage.
Instructions for Use
1. Conjugation Conditions
(1) The conjugation buffer should not contain primary amine groups, such as Tris buffer or glycine buffer.
(2) Borate buffer (pH 9.5) is recommended. Additionally, testing pH ranges from 7.0 (PBS) to 9.5 (borate) is suggested to determine optimal conditions.
(3) For IgG antibodies, the recommended conjugation ratio is IgG:Beads = 20–30 μg:1 mg. However, due to the diversity of proteins, screening different ratios is advised for optimal results.
(4) Conjugation is recommended at 37°C for 12–18 hours.
(5) Blocking is required after conjugation. The type and amount of blocking protein should be optimized (commonly used agents include BSA, casein, etc.). Blocking at 37°C for 6–16 hours is recommended.
2. Precautions
(1) Before use, thoroughly vortex the magnetic beads to ensure a uniform suspension.
(2) Drying or freezing can cause bead aggregation and may affect the activity of surface functional groups. Please adhere to the recommended storage conditions.
3. Recommended Conjugation Protocol
| Item | Details | Preparation Method |
| Conjugation Buffer A | 0.1M Borate Buffer, pH 9.5 | Dissolve 0.618 g H₃BO₃ (Boric Acid, MW 61.83) in 80 mL purified water. Adjust pH to 9.5 with 5M NaOH, then add purified water to 100 mL. |
| Conjugation Buffer B | Buffer A containing 3.0M Ammonium Sulfate, pH 9.5 | Dissolve 39.64 g (NH₄)₂SO₄ (Ammonium Sulfate, MW 132.14) in 80 mL Conjugation Buffer A. Adjust pH to 9.5 with 5M NaOH, then add Conjugation Buffer A to 100 mL. |
| Blocking Buffer | 50 mM Tris + 1.0% BSA + 0.1% Tween 20, pH 7.4 | Dissolve 0.606 g Tris (MW 121.14), 1.0 g BSA, and 0.1 g Tween 20 in 80 mL purified water. Adjust pH to 7.4, then add purified water to 100 mL. |
| Storage Buffer | 50 mM Tris + 1.0% BSA + 0.1% Tween 20, pH 7.4 | Same as above. |
4. Conjugation Method
(1) Thoroughly vortex the tosyl magnetic beads to suspend evenly, and transfer 10 mg to a centrifuge tube.
(2) Place the tube on a magnetic rack for 1–2 minutes and remove the supernatant.
(3) Add 1.0 mL of Conjugation Buffer A, vortex to disperse the beads, and remove the supernatant as in step (2).
(4) Repeat step (3) twice.
(5) Add 200 μg of antibody and supplement with Conjugation Buffer A to a total volume of 0.6 mL. Vortex to disperse the beads.
(6) Add 0.4 mL of Conjugation Buffer B and vortex to mix.
(7) Maintain the beads in suspension with mixing and react at 37°C for 16 hours. Remove the supernatant as in step (2).
(8) Add 1.0 mL of Blocking Buffer, vortex to disperse the beads. Maintain suspension with mixing and block at 37°C for 6–16 hours. Remove the supernatant as in step (2).
(9) Add 1.0 mL of Storage Buffer, vortex to disperse, and remove the supernatant as in step (2).
(10) Repeat step (9) twice.
(11) Add 1.0 mL of Storage Buffer, vortex to mix, resulting in a magnetic bead suspension at 10 mg/mL. Store at 2–8°C.
Application Examples
1. Comparison of Luminescence Values between T1506717-1.2μm and Imported Brand D in Anti-Müllerian Hormone (AMH) Detection
Conclusion:
Under identical bead conjugation and reagent detection conditions:
The luminescence values of T1506717-1.2μm magnetic beads in AMH detection are higher than those of the similar 1.0μm magnetic microspheres from Imported Brand D.
The luminescence values across three batches of T1506717-1.2μm show minimal variation, indicating a stable production process and low batch-to-batch variation.
2. Comparison of Luminescence Values between T1506717-2.8μm and Imported Brand D in Carcinoembryonic Antigen (CEA) Detection
Conclusion:
Under identical bead conjugation and reagent detection conditions:
The luminescence values of T1506717-2.8μm magnetic beads in CEA detection are higher than those of the similar 2.8μm magnetic microspheres from Imported Brand D.
The luminescence values across three batches of T1506717-2.8μm show minimal variation, indicating a stable production process and low batch-to-batch variation.
规格
规格或纯度
BioReagent, 用于化学发光, 10mg/mL
稳定性与储存
Store at 2-8℃ long term (48 months). Do not freeze. Keep the vial upright.
英文名称
Tosyl Magnetic Beads
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131302 |
分析证书 | T1506717 |
| ZJ26F0131301 |
分析证书 | T1506717 |
| ZJ26F0131221 |
分析证书 | T1506717 |
| ZJ26F0131222 |
分析证书 | T1506717 |
技术文档和文章
溶液计算器
常见问题
什么是「用于化学发光」？
「用于化学发光」表示该化学品经过纯化和检测，专用于专项分析技术。关键规格通常包括：常用检测波长处的受控紫外吸收、低不挥发残渣、受控的水分含量以及经验证的批间一致性。「用于化学发光」用于此工作流程中的流动相、样品制备和分析标准品。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C条件下保存。请勿冷冻。冷冻可能对本品造成不可逆的损坏。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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