| 产品名称 | 货号 | 表面基团 | 粒径 | 浓度 |
| 时间分辨荧光微球 | T1506734-A200nm | 羧基（COOH） | 200nm | 1% (10mg/mL) |
| 时间分辨荧光微球 | T1506734-A300nm | 羧基（COOH） | 300nm | 1% (10mg/mL) |
| 时间分辨荧光微球 | T1506734-B100nm | 链霉亲和素（SA） | 100nm | 1% (10mg/mL) |
| 时间分辨荧光微球 | T1506734-B200nm | 链霉亲和素（SA） | 200nm | 1% (10mg/mL) |
| 时间分辨荧光微球 | T1506734-B300nm | 链霉亲和素（SA） | 300nm | 1% (10mg/mL) |
| 名称 | 详情 |
| MES | 50mM MES, pH 6.0 |
| sulfo-NHS溶液 | 10mg/mL in MES |
| EDC溶液 | 10mg/mL in MES |
| 封闭液 | 50mM HEPES, 1% BSA, 0.1% ProClin 300, pH 8.0 |
| Product Name | Catalog Number | Surface Group | Particle Size | Concentration |
| Time Resolved Fluorescent Beads | T1506734-A200nm | Carboxyl (COOH) | 200nm | 1% (10mg/mL) |
| Time Resolved Fluorescent Beads | T1506734-A300nm | Carboxyl (COOH) | 300nm | 1% (10mg/mL) |
| Time Resolved Fluorescent Beads | T1506734-B100nm | Streptavidin (SA) | 100nm | 1% (10mg/mL) |
| Time Resolved Fluorescent Beads | T1506734-B200nm | Streptavidin (SA) | 200nm | 1% (10mg/mL) |
| Time Resolved Fluorescent Beads | T1506734-B300nm | Streptavidin (SA) | 300nm | 1% (10mg/mL) |
Product Features
Uniform particle size with CV ≤ 3% and low batch-to-batch variation.
High dye loading capacity and high fluorescence intensity.
Single-batch production capacity greater than 10 L, meeting most production demands.
Customized services are available.
Instructions for Use
One-Step Labeling Protocol
1. Solution Preparation
| Name | Details |
| MES | 50mM MES, pH 6.0 |
| sulfo-NHS Solution | 10mg/mL in MES |
| EDC Solution | 10mg/mL in MES |
| Blocking Buffer | 50mM HEPES, 1% BSA, 0.1% ProClin 300, pH 8.0 |
2. Recommended Labeling Protocol
(1) Activation: Take 100 μL of time-resolved latex microspheres (1% solid content). Dilute with 900 μL of MES Buffer and disperse by ultrasonication. Add 12 μL of sulfo-NHS Solution, mix well. Then add 10 μL of EDC Solution, mix well, and react at room temperature for 20 minutes. Centrifuge (RCF > 15,000 × g) for 5 minutes, remove the supernatant, and wash once by centrifugation with MES Buffer.
(2) Antibody Conjugation: Disperse the resulting microspheres in 200 μL of MES Buffer by ultrasonication. Add 120 μg of antibody (pre-diluted to 300 μL with MES Buffer), mix well, and react at room temperature for 2 hours.
(3) Blocking: Add 50 μL of Blocking Buffer, disperse by ultrasonication, and react at room temperature for at least 1 hour (overnight blocking is possible). Centrifuge (RCF > 15,000 × g) for 2 minutes, remove the supernatant, and wash once by centrifugation with Blocking Buffer.
(4) Storage: Add 100 μL of Storage Buffer (same as Blocking Buffer), disperse by ultrasonication, and store at 2–8°C.
Precautions
This product series should be stored sealed, protected from light, at 2–8°C. Do not freeze.
Before use, disperse the microspheres evenly using ultrasonication or a vortex mixer to ensure uniform dispersion.
危险品化学品经营许可证（带存储）