计算溶液所需的质量、体积或浓度。
台盼蓝染液（0.4%）
有货
为什么选择此级别
BioReagent,生物染色剂,适用于分析 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
是否无菌：是
台盼蓝染色液(0.4%)常用于检测细胞膜的完整性以及细胞是否存活。活细胞不会被染成蓝色，而死细胞会被染成淡蓝色。台盼蓝可被巨噬细胞吞噬，故也可用于巨噬细胞的活体染色剂。台盼蓝染色后，通过显微镜下直接计数或显微镜下拍照后计数，就可以对细胞存活率进行比较精确的定量，0.4%为最常用的浓度。
使用方法
1. 收集细胞：
如果收集贴壁细胞，应用胰蛋白酶-EDTA溶液消化细胞，收集细胞。如果收集悬浮细胞，则可以直接收集细胞。收集好的细胞在1000rpm离心5min，弃上清，用1mL PBS重新悬浮细胞沉淀。
2. 台盼蓝染色：
推荐向75μL细胞悬液中加入25μL台盼蓝储液(0.4%)轻轻混匀，终浓度为0.1% w/v。根据样品和仪器，终浓度范围参考从0.04%到0.2%（w/v）。室温染色3min(染色时间可适当延长，但不宜超过10min)。
3. 细胞计数：
吸取少量经过染色后的细胞，用血细胞计数板计数，镜下可观察到死细胞着蓝色并膨大无光泽，活细胞不着色，维持正常形态有光泽。
通常如果要比较精确地进行定量，每个细胞样品至少需要数500个细胞，数出蓝色细胞和细胞总数。细胞存活率计算公式如下：
细胞存活率＝(细胞总数－蓝色细胞数)/细胞总数×100%。
注意事项
1. 台盼蓝对人体有轻微毒性，请注意小心防护。
2. 注意凋亡小体偶尔也有台盼蓝拒染现象。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
结果展示
图 1
图 1. 台盼蓝染色液对 K562细胞存活率测试效果图。细胞1000rpm离心5分钟收集K562细胞，弃上清，用适量PBS重悬后，分别750μL细胞悬液加入250μL台盼蓝染色液（4×）或 900μL细胞悬液加入100μL台盼蓝染色液（10×）染色3min。台盼蓝染为蓝色的即为死亡细胞。
Trypan Blue Staining Solution (0.4%) is commonly used to assess cell membrane integrity and cell viability. Live cells remain unstained, while dead cells are stained light blue. Trypan Blue can be phagocytosed by macrophages, making it suitable for use as an in vivo stain for macrophages. After staining, cell viability can be accurately quantified by direct counting under a microscope or by counting from microscopic images. The concentration of 0.4% is the most commonly used concentration.
Protocol
1. Cell Collection
If collecting adherent cells, digest the cells with trypsin-EDTA solution, then harvest them. For suspension cells, cells can be collected directly. Centrifuge the collected cells at 1000 rpm for 5 minutes, discard the supernatant, and resuspend the cell pellet in 1 mL of PBS.
2. Trypan Blue Staining
It is recommended to add 25 μL of Trypan Blue stock solution (0.4%) to 75 μL of the cell suspension and mix gently. The final concentration will be 0.1% (w/v). Depending on the sample and instrument, the final concentration may range from 0.04% to 0.2% (w/v). Stain at room temperature for 3 minutes (staining time can be appropriately extended, but should not exceed 10 minutes).
3. Cell Counting
Transfer a small amount of the stained cell suspension to a hemocytometer for counting. Under the microscope, dead cells appear blue, swollen, and dull, while live cells remain unstained and exhibit normal morphology with a glossy appearance. For accurate quantification, at least 500 cells per sample should be counted. Count both the blue-stained (dead) cells and the total number of cells. Cell viability is calculated using the following formula:
Cell Viability (%) = (Total number of cells – Number of blue cells) / Total number of cells × 100%
Notes
1. Trypan Blue is slightly toxic to humans. Please take proper precautions to avoid exposure.
2. Note that apoptotic bodies may occasionally exhibit Trypan Blue exclusion as well.
3. For your safety and health, please wear a lab coat and disposable gloves during handling.
Results Presentation
Figure 1
Figure 1. Viability test of K562 cells using Trypan Blue staining. Cells were collected by centrifugation (1000 rpm, 5 min), resuspended in PBS. Stain for 3 minutes using either 750 μL cell suspension + 250 μL Trypan Blue (4×) or 900 μL cell suspension + 100 μL Trypan Blue (10×). Blue-stained cells were counted as dead cells.
规格
别名
台盼兰 | 锥虫蓝 | 曲利苯蓝 | 直接蓝
英文别名
Trypan Blue Solution | Blue cell viability dye
规格或纯度
BioReagent, 生物染色剂, 适用于分析, 0.4%
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Trypan Blue Solution, 0.4%
储存条件
室温
运输条件
常规运输
备注
此产品可替代 T1523203
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
应用
应用名称
稀释比例
Cell Counter
1/2-1/10
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939509 |
分析证书 | T1511149 |
| ZJ26F0939508 |
分析证书 | T1511149 |
| ZJ26F0939278 |
分析证书 | T1511149 |
| ZJ26F0939277 |
分析证书 | T1511149 |
| ZJ26F0938918 |
分析证书 | T1511149 |
| ZJ26F0737763 |
分析证书 | T1511149 |
| ZJ26F0737762 |
分析证书 | T1511149 |
溶液计算器
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 Suitable for Analysis 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）