蛋白酶抑制剂(Protease Inhibitor)是一类能与蛋白酶分子活性中心基团结合,使蛋白酶活力下降甚至消失,且不导致蛋白变性的物质。常见类型包括 EDTA、E-64、NaVO₃、Bestatin、Leupetin、Pepstatin A、Aprotinin 等,均可有效抑制蛋白降解,维持蛋白间原有相互作用。
胰蛋白酶抑制剂溶液 (1 mg/mL) 以胰蛋白酶抑制剂 Aprotinin 为主要成分,不含 EDTA,适用于哺乳动物组织及细胞的蛋白质提取,可高效获取目的蛋白,提取所得蛋白可用于 Western Blot、免疫共沉淀等后续实验。
操作步骤(仅供参考)
1. 开盖前进行低速离心,使管壁黏附的液体沉降至管底。
2. 按裂解液用量加入胰蛋白酶抑制剂溶液 (1 mg/mL),工作浓度控制在 1–10 μg/mL,常用浓度为 3–5 μg/mL。
注意事项
1. 胰蛋白酶抑制剂溶液 (1 mg/mL) 具有轻微刺激性,操作时需谨慎,同时避免反复冻融。
2. 为保障安全与健康,操作时请穿着实验服、佩戴一次性手套。
3. 本产品仅限科研使用,严禁用于其他用途。
Protease inhibitors are a class of substances that bind to groups at the active center of protease molecules, reducing or even eliminating protease activity without causing protein denaturation.Common types include EDTA, E-64, NaVO₃, Bestatin, Leupetin, Pepstatin A, Aprotinin, etc. All of them can effectively inhibit protein degradation and maintain the original interactions between proteins.
Trypsin inhibitor solution (1 mg/mL) is mainly composed of the trypsin inhibitor aprotinin and does not contain EDTA. It is suitable for protein extraction from mammalian tissues and cells, allowing efficient acquisition of target proteins. The extracted proteins can be used in subsequent experiments such as Western Blot and co-immunoprecipitation.
Operating Procedures (For Reference Only)
1. Centrifuge at low speed before opening the cap to settle the liquid adhering to the tube wall to the bottom of the tube.
2. Add Trypsin Inhibitor Solution (1 mg/mL) according to the volume of lysis buffer, with the working concentration controlled at 1–10 μg/mL, and the commonly used concentration being 3–5 μg/mL.
Precautions
1. Trypsin Inhibitor Solution (1 mg/mL) is slightly irritating. Handle with caution and avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. This product is for research use only and strictly prohibited for other purposes.