Tannase, whose full name is tannin acyl hydrolase (Tannase, EC 3.1.1.20), hydrolyzes the ester bonds and depside bonds in gallotannins to produce gallic acid and glucose.
Assay Principle: The antioxidant propyl gallate (PG) is used as the substrate for the tannase enzymatic reaction. The enzyme activity is calculated by measuring the change in optical density of the substrate PG before and after the reaction at 270 nm.
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