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植物叶绿体中磷酸丙糖异构酶是光合作用中参与calvin循环的重要酶。作用于磷酸甘油醛和磷酸二羟丙酮之间的转化，磷酸二羟丙酮能快速透过叶绿体的包膜进入细胞质，并在其中逐步转化为蔗糖。 测定原理：磷酸丙糖异构酶将磷酸二羟丙酮转化为3-磷酸甘油醛，3-磷酸甘油醛与NAD在3-磷酸甘油醛脱氢酶的作用下生成3-磷酸甘油酸和NADH，340nm处的吸光度变化反映了磷酸丙糖异构酶的活性的高低。
Triose phosphate isomerase in plant chloroplasts is an important enzyme involved in the Calvin cycle during photosynthesis. It catalyzes the interconversion between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Dihydroxyacetone phosphate can rapidly cross the chloroplast envelope into the cytoplasm, where it is gradually converted into sucrose. Determination Principle: Triose phosphate isomerase converts dihydroxyacetone phosphate into glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate, together with NAD⁺, is converted into 3-phosphoglycerate and NADH in the presence of glyceraldehyde-3-phosphate dehydrogenase. The change in absorbance at 340 nm reflects the activity level of triose phosphate isomerase.
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