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THL(EC 3.2.1.28)广泛存在于动物、植物、微生物和培养细胞中。海藻糖酶主要功能在于生物体分解海藻糖生成葡萄糖而直接用于能量供应。 测定原理:THL催化海藻糖产生葡萄糖,葡萄糖氧化酶催化葡萄糖氧化成葡萄糖酸,并产生过氧化氢;过氧化物酶催化过氧化氢氧化4-氨基安替比林偶联酚,生成有色化合物,在505 nm有特征吸收峰,以此反映THL活性。
THL (EC 3.2.1.28) is widely present in animals, plants, microorganisms, and cultured cells. The primary function of trehalase is to hydrolyze trehalose into glucose, which is directly utilized for energy supply. Determination Principle: THL catalyzes the hydrolysis of trehalose to produce glucose. Glucose oxidase catalyzes the oxidation of glucose to gluconic acid, producing hydrogen peroxide. Peroxidase catalyzes the oxidation of 4-aminoantipyrine coupled with phenol by hydrogen peroxide, forming a colored compound with a characteristic absorption peak at 505 nm, which reflects THL activity.