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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
茶多酚(Tea Polyphenols,TP)是茶叶中多酚类物质的总称,包括黄烷醇类、花色苷类、黄酮类、黄酮醇类和酚酸类等,是一类儿茶素为主体的黄酮化合物,儿茶素占60~80%,具有C6-C3-C6碳骨架结构,是一种重要的天然抗氧化物质,能够清除自由基。类物质茶多酚又称茶鞣或茶单宁,是形成茶叶色香味的主要成份之一,也是茶叶中有保健功能的主要成份之一,研究表明茶多酚等活性物质具解毒和抗辐射作用,能有效地阻止放射性物质侵入骨髓,并可使锶90和钴60迅速排出体外。 该试剂盒检测原理是以酒石酸铁为底物,利用茶多酚与酒石酸铁反应,生成稳定紫蓝色化合物,以酶标仪540nm处测定吸光度,在一定范围内吸光度与颜色深浅的变化成正比,与标准曲线比较进而计算茶多酚含量,主要用于测定植物组织、血清等样品中茶多酚含量,尤其适用于测定茶叶中茶多酚含量。本产品仅用于科研领域,不适用于临床诊断或其他用途。
Tea polyphenols (TP) are a general term for the polyphenolic substances in tea, including flavanols, anthocyanins, flavonoids, flavonols, and phenolic acids. They are a class of flavonoid compounds primarily composed of catechins, which account for 60-80%. These compounds feature a C6-C3-C6 carbon skeleton structure and are important natural antioxidants capable of scavenging free radicals. Also known as tea tannin, tea polyphenols are one of the main components contributing to the color, aroma, and flavor of tea, as well as a key component responsible for its health benefits. Research indicates that active substances such as tea polyphenols possess detoxifying and anti-radiation effects, effectively preventing radioactive substances from penetrating bones and facilitating the rapid excretion of Strontium-90 and Cobalt-60 from the body. The detection principle of this kit is based on using ferric tartrate as the substrate. Tea polyphenols react with ferric tartrate to form a stable violet-blue complex. The absorbance is measured at 540 nm using a microplate reader. Within a certain range, the absorbance value is proportional to the intensity of the color development. By comparing with a standard curve, the tea polyphenol content is calculated. This kit is primarily intended for measuring tea polyphenol content in samples such as plant tissues and serum, and is particularly suitable for determining tea polyphenol levels in tea leaves. This product is intended for research use only and is not for use in diagnostic procedures or other purposes.