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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
TH位于线粒体的内膜上,又称为线粒体复合体六,催化NADH+NADP⁺和NAD⁺+NADPH相互转化,调节线粒体NAD(H)和NADP(H)平衡。把逆向反应称为TH-2,催化NADPH和NAD⁺生成NADP⁺和NADH。 测定原理:NADH和NADPH均在340nm有特征吸收,因此TH催化的转氢反应不能导致340nm吸光度发生变化。用人工合成底物3-乙酰吡啶腺嘌呤二核苷酸(APAD⁺)替代NAD⁺,TH-2催化APAD⁺还原生成APADH,APADH在375nm有特征光吸收,测定375nm光吸收的增加速率,来计算TH-2活性。
TH is located on the inner mitochondrial membrane and is also known as mitochondrial complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH, thereby regulating the balance of mitochondrial NAD(H) and NADP(H). The reverse reaction is referred to as TH‑2, which catalyzes the conversion of NADPH and NAD⁺ to NADP⁺ and NADH. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide (APAD⁺) as a substitute for NAD⁺, TH‑2 catalyzes the reduction of APAD⁺ to APADH, which has a characteristic absorption peak at 375 nm. The TH‑2 activity is calculated by measuring the rate of increase in absorbance at 375 nm.