BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
单宁酶（单宁酯酰水解酶，Tannase，EC 3.1.1.20）可水解没食子酸单宁中的酯键和缩酚酸键，生成没食子酸和葡萄糖。该酶分布广泛，在食品加工、饲料加工、化妆品生产及皮革制作工艺中具有重要应用价值。本试剂盒采用绕丹宁比色法测定单宁酶活性：以没食子酸丙酯（PG）为底物，单宁酶水解PG释放没食子酸；在碱性条件下，没食子酸与绕丹宁形成红色复合物，该产物在520 nm处具有特征吸收峰。通过测定该波长下吸光值的增幅，结合标准曲线即可精准计算样品中单宁酶的活力大小。
Tannase (tannin acyl hydrolase, EC 3.1.1.20) hydrolyzes the ester and depside bonds in gallotannins to release gallic acid and glucose. This enzyme is widely distributed and has important applications in food processing, feed processing, cosmetics production, and leather manufacturing. This kit employs a rhodanine colorimetric method for the determination of tannase activity: using propyl gallate (PG) as the substrate, tannase hydrolyzes PG to release gallic acid. Under alkaline conditions, gallic acid reacts with rhodanine to form a red complex, which exhibits a characteristic absorbance peak at 520 nm. By measuring the increase in absorbance at this wavelength and referencing a standard curve, the tannase activity in the sample can be precisely calculated.
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