计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
6-磷酸海藻糖酯酶(trehalose-6-phosphate phosphatase,TPP,EC 3.1.3.12)是海藻糖合成的关键酶之一,催化海藻糖-6-磷酸脱磷酸生成海藻糖。本试剂盒利用TPP催化底物海藻糖-6-磷酸生成海藻糖,海藻糖经海藻糖酶水解为葡萄糖,葡萄糖在葡萄糖氧化酶作用下与特异性显色剂反应生成有色产物,通过检测该产物在520 nm处的吸光度,即可计算TPP的活性。
Trehalose-6-phosphate phosphatase (TPP, EC 3.1.3.12) is one of the key enzymes in trehalose biosynthesis, catalyzing the dephosphorylation of trehalose-6-phosphate to generate trehalose. This kit employs TPP to catalyze the conversion of the substrate trehalose-6-phosphate into trehalose. The resulting trehalose is subsequently hydrolyzed to glucose by trehalase, and the glucose then reacts with a specific chromogenic reagent under the action of glucose oxidase to form a colored product. By measuring the absorbance of this product at 520 nm, the activity of TPP can be calculated.