计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
磷酸丙糖异构酶(EC 5.3.1.1,TPI/TIM)是糖酵解途径中的关键酶,催化磷酸二羟丙酮与3-磷酸甘油醛之间的可逆异构化,维持二者的代谢平衡。TPI连接糖酵解、戊糖磷酸途径与脂质代谢,且以稳定的同型二聚体形式广泛存在于几乎所有生物中。 本试剂盒提供一种快速、简单且灵敏的检测方法。TPI催化磷酸二羟丙酮转化为3-磷酸甘油醛,产物与偶联酶混合物及特异性显色探针反应,生成在450 nm处有最大吸收的有色物质。通过测定450 nm处吸光度的增加速率,即可计算TPI活性。
Triosephosphate isomerase (EC 5.3.1.1, TPI/TIM) is a key enzyme in the glycolytic pathway, catalyzing the reversible isomerization between dihydroxyacetone phosphate and glyceraldehyde 3‑phosphate, thereby maintaining the metabolic balance between these two metabolites. TPI connects glycolysis, the pentose phosphate pathway, and lipid metabolism, and is widely present in virtually all organisms as a stable homodimer. This kit provides a rapid, simple, and sensitive detection method. TPI catalyzes the conversion of dihydroxyacetone phosphate to glyceraldehyde 3‑phosphate, and the product reacts with a coupled enzyme mixture and a specific chromogenic probe to generate a colored substance with maximum absorbance at 450 nm. By measuring the rate of increase in absorbance at 450 nm, the TPI activity can be calculated.