BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
超氧化物歧化酶（Superoxide Dismutase, SOD）是一种含金属辅基的酶，能催化超氧化物阴离子发生歧化反应，生成过氧化氢（H₂O₂）和氧气（O₂），是生物体内重要的抗氧化酶。由于超氧自由基不稳定、寿命极短，SOD活性通常采用间接方法测定，利用各种显色反应来反映酶活力，常用显色剂包括NBT（四氮唑蓝）、WST-1、WST-8等。 本产品采用经典的氮蓝四唑（NBT）显色法。检测原理为：通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子（O₂⁻·），该阴离子能将氮蓝四唑还原为蓝色的甲臜（formazan），后者在560 nm处具有强吸收。而SOD可清除超氧阴离子，从而抑制甲臜的生成。反应液蓝色越深，表明SOD活性越低；反之，蓝色越浅则酶活性越高。据此，通过酶标仪或分光光度计进行比色分析，即可计算出超氧化物歧化酶的活性水平。本产品可用于检测细胞或组织匀浆液上清、全血、红细胞抽提物、血清等样品中的SOD活性。本试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
Superoxide Dismutase (SOD) is a metalloenzyme that catalyzes the dismutation of superoxide anions into hydrogen peroxide (H₂O₂) and oxygen (O₂), serving as a critical antioxidant enzyme in living organisms. Due to the instability and extremely short half‑life of superoxide radicals, SOD activity is typically measured indirectly using various chromogenic reactions, with commonly used chromogenic reagents including NBT (nitro blue tetrazolium), WST‑1, and WST‑8. This product employs the classical nitro blue tetrazolium (NBT) colorimetric method. The detection principle is as follows: superoxide anions (O₂⁻·) are generated by the xanthine and xanthine oxidase reaction system. These anions reduce NBT to blue formazan, which exhibits strong absorbance at 560 nm. SOD scavenges superoxide anions, thereby inhibiting formazan formation. A darker blue color in the reaction solution indicates lower SOD activity, whereas a lighter blue color indicates higher enzyme activity. Accordingly, the SOD activity level can be calculated by colorimetric analysis using a microplate reader or spectrophotometer. This product is suitable for measuring SOD activity in samples such as cell or tissue homogenate supernatants, whole blood, erythrocyte extracts, and serum. This kit is intended for research use only and is not suitable for clinical diagnosis or other applications.
危险品化学品经营许可证（带存储）