计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用酶偶联比色法测定总胆固醇含量。胆固醇酯酶催化胆固醇酯水解生成游离胆固醇(FC)和游离脂肪酸(FFA);胆固醇氧化酶催化游离胆固醇氧化生成Δ⁴-胆甾烯酮和H₂O₂;过氧化物酶催化H₂O₂氧化4-氨基安替比林与苯胺类似物,生成紫色化合物,该产物在550 nm处具有特征吸收峰。通过测定该波长下吸光度的变化,即可定量检测样品中总胆固醇的含量。
This kit employs an enzyme‑coupled colorimetric method for the determination of total cholesterol (TC) content. Cholesterol esterase catalyzes the hydrolysis of cholesteryl esters to free cholesterol (FC) and free fatty acids (FFA). Cholesterol oxidase catalyzes the oxidation of free cholesterol to generate Δ⁴‑cholestenone and hydrogen peroxide (H₂O₂). Subsequently, peroxidase (POD) catalyzes the oxidation of 4‑aminoantipyrine and an aniline analog by H₂O₂ to produce a purple compound, which exhibits a characteristic absorption peak at 550 nm. By measuring the change in absorbance at this wavelength, the total cholesterol content in the sample can be quantitatively determined.