计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
多糖是一类由醛糖或酮糖通过糖苷键连接而成的天然高分子多聚物,广泛分布于生物体内,是维持生命活动正常运转的重要生物大分子之一。本试剂盒采用水提醇沉法提取样品中的总多糖,再利用苯酚-硫酸法进行测定:多糖在浓硫酸作用下水解生成单糖,并迅速脱水生成糠醛类化合物,后者与苯酚反应生成橙黄色有色物质,该产物在490 nm处具有特征吸收峰,通过测定吸光度即可定量计算样品中总多糖含量。
Polysaccharides are natural high‑molecular‑weight polymers composed of aldoses or ketoses linked by glycosidic bonds. They are widely distributed in living organisms and are one of the important biological macromolecules that sustain normal physiological functions. This kit employs a water extraction‑alcohol precipitation method to extract total polysaccharides from samples, followed by determination using the phenol‑sulfuric acid method. The principle is as follows: Polysaccharides are hydrolyzed to monosaccharides under the action of concentrated sulfuric acid, which are then rapidly dehydrated to furfural‑type compounds. These compounds react with phenol to produce an orange‑yellow colored product that exhibits a characteristic absorption peak at 490 nm. The total polysaccharide content in the sample can be quantitatively determined by measuring the absorbance.