BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
总谷胱甘肽（T-GSH）包括还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）。GSSG又称二硫代谷胱甘肽，由两分子GSH氧化而成。谷胱甘肽具有维持正常免疫功能、抗氧化及整合解毒等作用。GSSG可在谷胱甘肽还原酶催化下还原为GSH，因此机体中谷胱甘肽主要以还原型形式存在。测定细胞内GSH和GSSG含量及GSH/GSSG比值，能够有效反映细胞所处的氧化还原状态，是谷胱甘肽氧化还原循环的核心指标之一。 本试剂盒的检测原理如下：谷胱甘肽还原酶（GR）将GSSG还原为GSH；GSH与5,5-二硫双（2-硝基苯甲酸）（DTNB）反应生成黄色的2-硝基-5-巯基苯甲酸。通过测定412 nm处吸光值的变化，即可计算样本中总谷胱甘肽含量。若使用适当的封闭剂抑制样本中原有的GSH，然后按上述反应测定，则可获得GSSG含量。从总谷胱甘肽含量中减去GSSG含量，即可计算出样本中GSH的含量。
Total glutathione (T-GSH) includes reduced glutathione (GSH) and oxidized glutathione (GSSG). GSSG, also known as glutathione disulfide, is formed by the oxidation of two GSH molecules. Glutathione plays important roles in maintaining normal immune function, antioxidation, and chelation detoxification. GSSG can be reduced back to GSH by glutathione reductase, so glutathione in the body predominantly exists in the reduced form. Determining the intracellular levels of GSH and GSSG, as well as the GSH/GSSG ratio, effectively reflects the cellular redox status and is one of the core indicators of the glutathione redox cycle. The detection principle of this kit is as follows: glutathione reductase (GR) reduces GSSG to GSH; GSH then reacts with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to generate yellow 2-nitro-5-mercaptobenzoic acid (TNB). By measuring the change in absorbance at 412 nm, the total glutathione content in the sample can be calculated. If an appropriate blocking agent is used to inhibit the pre-existing GSH in the sample and the above reaction is then performed, the GSSG content can be obtained. By subtracting the GSSG content from the total glutathione content, the GSH content in the sample can be calculated.
危险品化学品经营许可证（带存储）