计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无菌过滤,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品用于 SDS-PAGE 电泳浓缩胶配制的缓冲液,由0.22μm 膜过滤纯化。组分包括0.4%SDS,0.5 M Tris 碱。配胶时可以可以按说明书直接使用。
注意事项:
1 过硫酸铵配制成 10%溶液后应当-20℃保存。应尽量减少室温存放时间以防失效。
2 TEMED 有轻微刺激性气味,使用时注意防护。
3 配胶用丙烯酰胺有神经毒性,可通过皮肤吸收,其作用具有积累性,配制时应戴手套。聚丙烯酰胺(聚合后)无毒,但也应谨慎操作,因为有可能含有少量的未聚合成份。
4 升温可以加快凝胶的聚合时间。
5 为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
可根据配制丙烯酰胺凝胶(PAGE 胶),包括 SDS-PAGE 胶等配方表使用本产品。
配制 SDS-PAGE 浓缩胶:
| 凝胶体积 | 蒸馏水 | Acr-Bis(30%,29:1) | 浓缩胶缓冲液 | 10%APS | TEMED |
| 2 mL | 1.14 | 0.34 | 0.5 | 0.02 | 0.004 |
| 4 mL | 2.28 | 0.68 | 1 | 0.04 | 0.008 |
| 6 mL | 3.42 | 1.02 | 1.5 | 0.06 | 0.012 |
| 8 mL | 4.56 | 1.36 | 2 | 0.08 | 0.016 |
Is it sterile: Yes
This product is a buffer for preparing stacking gel in SDS-PAGE electrophoresis and purified by filtration through a 0.22 μm membrane. Components: 0.4% SDS, 0.5 M Tris base. It can be used directly for gel casting following the instructions.
Precautions
1.10% ammonium persulfate (APS) solution should be stored at −20 ℃. Minimize storage at room temperature to prevent inactivation.
2.TEMED has a slight pungent odor; wear appropriate protective equipment during handling.
3.Acrylamide for gel casting is neurotoxic, absorbable through skin with cumulative toxic effects. Wear gloves while preparing gels. Polymerized polyacrylamide is non-toxic, but handle cautiously as trace unpolymerized monomers may remain.
4.Elevated temperature accelerates gel polymerization.
5.For your safety and health, wear a lab coat and disposable gloves during operation.
Instructions for Use
This product can be used according to formulations for polyacrylamide gels (PAGE), including SDS-PAGE gels.
For preparation of SDS-PAGE stacking gel:
| Gel Volume | Distilled Water | Acr-Bis (30%, 29:1) | Stacking Gel Buffer | 10% APS | TEMED |
| 2 mL | 1.14 | 0.34 | 0.5 | 0.02 | 0.004 |
| 4 mL | 2.28 | 0.68 | 1 | 0.04 | 0.008 |
| 6 mL | 3.42 | 1.02 | 1.5 | 0.06 | 0.012 |
| 8 mL | 4.56 | 1.36 | 2 | 0.08 | 0.016 |
| pH | 6.8 |
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我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 Suitable for electrophoresis 级别指南 → 查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 →