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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品用途:用于各种苛养微生物尤其是布氏杆菌的培养。 配制方法:称取本品39.0 g,溶于1 L蒸馏水或去离子水中,加热至沸腾,使完全溶解。分装于适宜容器中,于116 ℃高压灭菌30分钟。灭菌完毕后,摇匀,以防琼脂沉积于容器底部而凝固,备用。若需制备血平板,则于灭菌后冷却至50~55 ℃,在无菌操作下加入5%预先保温的脱纤维羊血,轻轻混匀,倾注平板。 配方:/升 胰蛋白月示 20.0g 氯化钠 5.0g 葡萄糖 1.0g 琼脂 13.0g pH 6.9±0.1(25℃)
Product application: For the cultivation of various fastidious microorganisms, especially Brucella. Preparation method: Weigh 39.0 g of this product, dissolve in 1 L of distilled or deionized water, heat to boiling until completely dissolved. Dispense into suitable containers and autoclave at 116°C for 30 minutes. After sterilization, shake well to prevent agar from settling and solidifying at the bottom of the container, then set aside for use. If preparing blood agar plates, after sterilization cool to 50–55°C, add 5% pre-warmed defibrinated sheep blood under aseptic conditions, mix gently, and pour into plates. Formula: /L Tryptose 20.0 g Sodium chloride 5.0 g Glucose 1.0 g Agar 13.0 g pH 6.9 ± 0.1 (25°C)